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Type: Artigo de periódico
Title: Effect of magnesium chloride and guanidinum chloride on the extraction of components of extracellular matrix from chicken cartilage
Author: Arcanjo, Kátia Denise Souza
Gomes, Laurecir
Pimentel, Edson Rosa
Abstract: In order to evaluate the effect of chaotropic agents on proteoglycan and non-collagenous proteins, chicken xiphoid cartilage was treated with guanidine-HCI and MgCl2 in different concentrations (1M to 5M), and different periods of time (12, 24, 48 and 72hr). The maximum yield of uronic acid was obtained with 3M MgCl2 (73.3 per cent). Concentrations of 4M and 5M of MgCl2 showed that much less uronic acid was removed, 55.3 per cent and 38.1 respectively. Extraction with 3M MgCl2 and 3M guanidine-HCl resulted better efficiency when performed for 48 hr. Analysis by SDS-PAGE of the extracts obtained with guanidine-HCl and MgCl, in different concentrations pointed out that most components are equally removed with the two solvents, showing that the extraction with MgCl2 is an alternative assay to remove non-collagenous proteins from extracellular matrix.
Subject: proteoglycans
non-collagenous proteins
extracelullar matrix
Editor: Instituto Oswaldo Cruz, Ministério da Saúde
Citation: Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 89, n. 1, p. 93-97, 1994.
Rights: aberto
Identifier DOI: 10.1590/S0074-02761994000100016
Date Issue: 1-Mar-1994
Appears in Collections:Unicamp - Artigos e Outros Documentos

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