Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/21400
Type: Artigo de periódico
Title: INTRACELLULAR Leishmania amazonensis KILLING INDUCED BY THE GUANINE NUCLEOSIDE 8-BROMOGUANOSINE
Author: GIORGIO, Selma
BARÃO, Sandra C.
Abstract: In this study we investigated the effect of 8-Bromoguanosine, an immunostimulatory compound, on the cytotoxicity of macrophages against Leishmania amazonensis in an in vitro system. The results showed that macrophages treated with 8-Bromoguanosine before or after infection are capable to reduce parasite load, as monitored by the number of amastigotes per macrophage and the percentage of infected cells (i.e. phagocytic index). Since 8-Bromoguanosine was not directly toxic to the promastigotes, it was concluded that the ribonucleoside induced macrophage activation. Presumably, 8-Bromoguanosine primed macrophages by inducing interferon alpha and beta which ultimately led to L. amazonensis amastigote killing. The results suggest that guanine ribonucleosides may be useful to treat infections with intracellular pathogens.
Neste trabalho, nós investigamos o efeito da 8-Bromoguanosina, um composto imunoestimulador, na citotoxicidade de macrófagos infectados com Leishmania amazonensis em um sistema in vitro. Os resultados mostraram que macrófagos tratados com 8-Bromoguanosina pré- ou pós- infecção foram capazes de reduzir a carga parasitária, monitorada pelo número de amastigotas por macrófago e a percentagem de células infectadas (i.e. índice fagocítico). Sendo a 8-Bromoguanosina inócua para promastigotas, concluímos que o composto induz ativação celular. Os macrófagos produziriam interferon alfa e beta e teriam seus mecanismos leishmanicidas estimulados. Esses resultados sugerem que compostos como a 8-Bromoguanosina (ribonucleosideos de guanina) podem auxiliar no tratamento contra patógenos intracelulares.
Subject: Leishmania amazonensis
Macrophage
Guanine ribonucleosides
8-Bromoguanosine
Immunostimulators
Editor: Instituto de Medicina Tropical
Rights: aberto
Identifier DOI: 10.1590/S0036-46651998000400006
Address: http://dx.doi.org/10.1590/S0036-46651998000400006
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651998000400006
Date Issue: 1-Jul-1998
Appears in Collections:Unicamp - Artigos e Outros Documentos

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