Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/21390
Type: Artigo de periódico
Title: Murine virus contaminant of Trypanosoma cruzi experimental infection
Title Alternative: Virus murino como contaminante na infecção experimental pelo Trypanosoma cruzi
Author: Rangel, Humberto de A.
Verinaud, Liana
Camargo, Irineu J. B.
Gilioli, Rovilson
Sakurada, Julia K.
Abstract: The possibility that some virus contaminants could be altering host response to Trypanosoma cruzi experimental infection was investigated. Data obtained showed that CBA/J mice infected with stocks of parasite maintained in mice (Y1UEC) presented higher level of parasitemia and shorter survival times than those infected with a stock (Y1TC) which was also maintained in mice but had been previously passaged in cell culture. Mouse antibody production tests, performed with the filtered plasma of mice infected with Y1UEC, indicated the presence of mouse hepatitis virus (MHV) while no virus was detected when testing the plasma of Y1TC infected mice. Filtered plasma of Y1EUC infected mice was shown to contain a factor able to enhance the level of parasitemia and to reduce the mean survival time of mice challenged with 10(5) Y1TC. This factor, that could be serially passaged to naïve mice was shown to be a coronavirus by neutralization tests.
A possibilidade de que alguma contaminação por virus poderia estar alterando a resposta do hospedeiro à infecção experimental por Trypanosoma cruzi foi investigada. Os dados obtidos mostraram que camundongos CBA/J infectados com estoques de parasitos mantidos em camundongos (Y1UEC) apresentavam maior parasitemia e menor período de sobrevivência do que os infectados com um estoque (Y1TC) que também foi mantido em animal mas tinha sido previamente passado em cultura de células. Testes de produção de anticorpos em camundongos (Map tests) realizados com o plasma fliltrado de camundongos infectados com Y1UEC indicaram a presença do virus da hepatite do camundongo (MHV) enquanto nenhum virus foi detectado no plasma de animais infectados com Y1TC. O plasma filtrado obtido de camundongos infectados com Y1UEC continha um fator capaz de aumentar o nível de parasitemia e reduzir o tempo médio de sobrevivencia dos camundongos desafiados com 10(5) Y1TC. Testes de neutralização mostraram que este fator que podia ser passado seriadamente para camundongos era um coronavirus.
Subject: Chagas' disease
Trypanosoma cruzi
Immune response
Coronavirus
Editor: Instituto de Medicina Tropical
Rights: aberto
Identifier DOI: 10.1590/S0036-46651994000500006
Address: http://dx.doi.org/10.1590/S0036-46651994000500006
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651994000500006
Date Issue: 1-Oct-1994
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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