Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/2095
Type: Artigo de periódico
Title: Second Harmonic Generation Microscopy Used to Evaluate the Effect of the Dimethyl Sulfoxide Cryopreservation Process in Collagen Fibers of Differentiated Chondrocytes
Author: Andreoli-Risso, M. F.
Duarte, A. S. S.
Ribeiro, T. B.
Bordeaux-Rego, P.
Luzo, A.
Baratti, M. O.
Adur, J.
de Thomaz, A. A.
Pelegati, V. B.
Carvalho, H. F.
Cesar, C. L.
Kharmadayan, P.
Costa, F. F.
Olalla-Saad, S. T.
Abstract: Cartilaginous lesions are a significant public health problem and the use of adult stem cells represents a promising therapy for this condition. Cryopreservation confers many advantages for practitioners engaged in cell-based therapies. However, conventional slow freezing has always been associated with damage and mortality due to intracellular ice formation, cryoprotectant toxicity, and dehydration. The aim of this work is to observe the effect of the usual Dimethyl Sulfoxide (DMSO) cryopreservation process on the architecture of the collagen fiber network of chondrogenic cells from mesenchymal stem cells by Second Harmonic Generation (SHG) microscopy. To perform this study we used Mesenchymal Stem Cells (MSC) derived from adipose tissue which presents the capacity to differentiate into other lineages such as osteogenic, adipogenic and chondrogenic lineages. Mesenchymal stem cells obtained after liposuction were isolated digested by collagenase type I and characterization was carried out by differentiation of mesodermic lineages, and flow cytometry using specific markers. The isolated MSCs were cryopreserved by the DMSO technique and the chondrogenic differentiation was carried out using the micromass technique. We then compared the cryopreserved vs non-cryopreserved collagen fibers which are naturally formed during the differentiation process. We observed that non-cryopreserved MSCs presented a directional trend in the collagen fibers formed which was absent in the cryopreserved MSCs. We confirmed this trend quantitatively by the aspect ratio obtained by Fast Fourier Transform which was 0.76 for cryopreserved and 0.52 for non-cryopreserved MSCs, a statistical significant difference.
Subject: Second Harmonic Generation (SHG) microscopy
Mesenchymal Stem Cells
chondrogenic differentiation
collagen
Dimethyl Sulfoxide (DMSO)
Editor: Spie-Int Soc Optical Engineering
Rights: fechado
Identifier DOI: 10.1117/12.909284
Date Issue: 2012
Appears in Collections:FCM - Artigos e Materiais de Revistas Científicas

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