Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/2039
Type: Artigo de periódico
Title: Immobilization of marine fungi on silica gel, silica xerogel and chitosan for biocatalytic reduction of ketones
Author: Rocha, Lenilson Coutinho
de Souza, Adriano Lopes
Rodrigues Filho, Ubirajara Pereira
Campana Filho, Sergio Paulo
Sette, Lara Duraes
Meleiro Porto, Andre Luiz
Abstract: The scanning electron microscopy (SEM) analysis showed that whole living hyphal of marine fungi Aspergillus sclerotiorum CBMAI 849 and Penicillium citrinum CBMAI 1186 were immobilized on support matrices of silica gel, silica xerogel and/or chitosan. P. citrinum immobilized on chitosan catalyzed the quantitative reduction of 1-(4-methoxyphenyl)-ethanone (1) to the enantiomer (S)-1-(4-methoxyphenyl)-ethanol (3b), with excellent enantioselectivity (ee > 99%, yield = 95%). Interestingly, ketone 1 was reduced with moderate selectivity and conversion to alcohol 3b (ee = 69%, c 40%) by the free mycelium of P. citrinum. This free mycelium of P. citrinum catalyzed the production of the (R)-alcohol 3a, the antipode of the alcohol produced by the immobilized cells. P. citrinum immobilized on chitosan also catalyzed the bioreduction of 2-chloro-1-phenylethanone (2) to 2-chloro-1-phenylethanol (4a,b), but in this case without optical selectivity. These results showed that biocatalytic reduction of ketones by immobilization hyphal of marine fungi depends on the xenobiotic substrate and the support matrix used. (c) 2012 Elsevier B.V. All rights reserved.
Subject: Biocatalysis
Penicillium citrinum
Aspergillus sclerotiorum
Whole living cells
Scanning electron microscopy
Editor: Elsevier
Citation: Journal of Molecular Catalysis B: Enzymatic. Elsevier, v.84, p.160-165, 2012
Rights: fechado
Identifier DOI: 10.1016/j.molcatb.2012.05.025
Date Issue: 2012
Appears in Collections:CPQBA - Artigos e Outros Documentos

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