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Type: Artigo de periódico
Title: Cromatografia de afinidade por íons metálicos imobilizados (IMAC) de biomoléculas: aspectos fundamentais e aplicações tecnológicas
Title Alternative: Immobilized metal-ion affinity chromatography (IMAC) of biomolecules: fundamental aspects and technological applications
Author: Bresolin, Igor Tadeu Lazzarotto
Miranda, Everson Alves
Bueno, Sônia Maria Alves
Abstract: Immobilized Metal Ion Affinity Cromatography - IMAC - is a group-specific based adsorption applied to the purification and structure-function studies of proteins and nucleic acids. The adsorption is based on coordination between a metal ion chelated on the surface of a solid matrix and electron donor groups at the surface of the biomolecule. IMAC is a highly selective, low cost, and easily scaled-up technique being used in research and commercial operations. A separation process can be designed for a specific molecule by just selecting an appropriate metal ion, chelating agent, and operational conditions such as pH, ionic strength, and buffer type.
Subject: immobilized metal-ion affinity chromatography
Editor: Sociedade Brasileira de Química
Citation: Química Nova. Sociedade Brasileira de Química, v. 32, n. 5, p. 1288-1296, 2009.
Rights: aberto
Identifier DOI: 10.1590/S0100-40422009000500035
Date Issue: 1-Jan-2009
Appears in Collections:Unicamp - Artigos e Outros Documentos

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