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Type: Artigo de periódico
Title: Extraction, purification and biochemical characterization of a peroxidase from Copaifera langsdorffii leaves
Author: Maciel, Hermelinda Penha Freire
Gouvêa, Cibele Marli Cação Paiva
Toyama, Marcos
Smolka, Marcus
Marangoni, Sergio
Pastore, Gláucia Maria
Abstract: The aim of this work is to obtain, purify and characterize biochemically a peroxidase from Copaifera langsdorffii leaves (COP). COP was obtained by acetone precipitation followed by ion-exchange chromatography. Purification yielded 3.5% of peroxidase with the purification factor of 46.86. The COP optimum pH is 6.0 and the temperature is 35 ºC. COP was stable in the pH range of 4.5 to 9.3 and at temperatures below 50.0 ºC. The apparent Michaelis-Menten constants (Km) for guaiacol and H2O2 were 0.04 mM and 0.39 mM respectively. Enzyme turnover was 0.075 s-1 for guaiacol and 0.28 s-1 for hydrogen peroxide. Copaifera langsdorffii leaves showed to be a rich source of active peroxidase (COP) during the whole year. COP could replace HRP, the most used peroxidase, in analytical determinations and treatment of industrial effluents at low cost.
Subject: Copaifera langsdorffii
vegetal extract
Editor: Sociedade Brasileira de Química
Citation: Química Nova. Sociedade Brasileira de Química, v. 30, n. 5, p. 1067-1071, 2007.
Rights: aberto
Identifier DOI: 10.1590/S0100-40422007000500003
Date Issue: 1-Oct-2007
Appears in Collections:Unicamp - Artigos e Outros Documentos

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