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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleImatinib Restores Vasp Activity And Its Interaction With Zyxin In Bcr-abl Leukemic Cells.pt_BR
dc.contributor.authorBernusso, Vanessa Apt_BR
dc.contributor.authorMachado-Neto, João Apt_BR
dc.contributor.authorPericole, Fernando Vpt_BR
dc.contributor.authorVieira, Karla Ppt_BR
dc.contributor.authorDuarte, Adriana S Spt_BR
dc.contributor.authorTraina, Fabiolapt_BR
dc.contributor.authorHansen, Marc Dpt_BR
dc.contributor.authorOlalla Saad, Sara Tpt_BR
dc.contributor.authorBarcellos, Karin S Apt_BR
unicamp.authorVanessa A Bernusso, Hematology and Hemotherapy Center-University of Campinas/Hemocentro-Unicamp, Instituto Nacional de Ciência e Tecnologia do Sangue, Campinas, São Paulo, Brazil.pt_BR
unicamp.authorJoão A Machado-Neto, Hematology and Hemotherapy Center-University of Campinas/Hemocentro-Unicamp, Instituto Nacional de Ciência e Tecnologia do Sangue, Campinas, São Paulo, Brazil.pt_BR
unicamp.authorFernando V Pericole, Hematology and Hemotherapy Center-University of Campinas/Hemocentro-Unicamp, Instituto Nacional de Ciência e Tecnologia do Sangue, Campinas, São Paulo, Brazil.pt_BR
unicamp.authorKarla P Vieira, Hematology and Hemotherapy Center-University of Campinas/Hemocentro-Unicamp, Instituto Nacional de Ciência e Tecnologia do Sangue, Campinas, São Paulo, Brazil.pt_BR
unicamp.authorAdriana S S Duarte, Hematology and Hemotherapy Center-University of Campinas/Hemocentro-Unicamp, Instituto Nacional de Ciência e Tecnologia do Sangue, Campinas, São Paulo, Brazil.pt_BR
unicamp.authorFabiola Traina, Hematology and Hemotherapy Center-University of Campinas/Hemocentro-Unicamp, Instituto Nacional de Ciência e Tecnologia do Sangue, Campinas, São Paulo, Brazil.pt_BR
unicamp.authorSara T Olalla Saad, Hematology and Hemotherapy Center-University of Campinas/Hemocentro-Unicamp, Instituto Nacional de Ciência e Tecnologia do Sangue, Campinas, São Paulo, Brazil.pt_BR
unicamp.authorKarin S A Barcellos, Hematology and Hemotherapy Center-University of Campinas/Hemocentro-Unicamp, Instituto Nacional de Ciência e Tecnologia do Sangue, Campinas, São Paulo, Brazil. Electronic address: kabarcellos@gmail.com.pt_BR
unicamp.author.externalMarc D Hansen, Department of Physiology and Developmental Biology, Brigham Young University, Provo, UT, USA.pt
dc.subjectApoptosispt_BR
dc.subjectBcr–ablpt_BR
dc.subjectLeukemiapt_BR
dc.subjectVasppt_BR
dc.subjectVasodilator-stimulated Phosphoproteinpt_BR
dc.subjectZyxinpt_BR
dc.description.abstractVasodilator-stimulated phosphoprotein (VASP) and Zyxin are interacting proteins involved in cellular adhesion and motility. PKA phosphorylates VASP at serine 157, regulating VASP cellular functions. VASP interacts with ABL and is a substrate of the BCR-ABL oncoprotein. The presence of BCR-ABL protein drives oncogenesis in patients with chronic myeloid leukemia (CML) due to a constitutive activation of tyrosine kinase activity. However, the function of VASP and Zyxin in BCR-ABL pathway and the role of VASP in CML cells remain unknown. In vitro experiments using K562 cells showed the involvement of VASP in BCR-ABL signaling. VASP and Zyxin inhibition decreased the expression of anti-apoptotic proteins, BCL2 and BCL-XL. Imatinib induced an increase in phosphorylation at Ser157 of VASP and decreased VASP and BCR-ABL interaction. VASP did not interact with Zyxin in K562 cells; however, after Imatinib treatment, this interaction was restored. Corroborating our data, we demonstrated the absence of phosphorylation at Ser157 in VASP in the bone marrow of CML patients, in contrast to healthy donors. Phosphorylation of VASP on Ser157 was restored in Imatinib responsive patients though not in the resistant patients. Therefore, we herein identified a possible role of VASP in CML pathogenesis, through the regulation of BCR-ABL effector proteins or the absence of phosphorylation at Ser157 in VASP.en
dc.relation.ispartofBiochimica Et Biophysica Actapt_BR
dc.relation.ispartofabbreviationBiochim. Biophys. Actapt_BR
dc.date.issued2015-Febpt_BR
dc.identifier.citationBiochimica Et Biophysica Acta. v. 1853, n. 2, p. 388-95, 2015-Feb.pt_BR
dc.language.isoengpt_BR
dc.description.volume1853pt_BR
dc.description.firstpage388-95pt_BR
dc.rightsfechadopt_BR
dc.rights.holderCopyright © 2014 Elsevier B.V. All rights reserved.pt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn0006-3002pt_BR
dc.identifier.doi10.1016/j.bbamcr.2014.11.008pt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/25450971pt_BR
dc.date.available2015-11-27T13:46:42Z-
dc.date.accessioned2015-11-27T13:46:42Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T13:46:42Z (GMT). No. of bitstreams: 1 pmed_25450971.pdf: 1240758 bytes, checksum: f0079b900629c98354ff3965e2e9740f (MD5) Previous issue date: 2015en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/202304-
dc.identifier.idPubmed25450971pt_BR
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