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Type: Artigo de periódico
Title: In Vivo Determination Of The Volatile Metabolites Of Saprotroph Fungi By Comprehensive Two-dimensional Gas Chromatography.
Author: de Lima, Paula Feliciano
Furlan, Mayra Fontes
Ribeiro, Fabiana Alves de Lima
Pascholati, Sérgio Florentino
Augusto, Fabio
Abstract: In this work, we discuss the use of multi-way principal component analysis combined with comprehensive two-dimensional gas chromatography to study the volatile metabolites of the saprophytic fungus Memnoniella sp. isolated in vivo by headspace solid-phase microextraction. This fungus has been identified as having the ability to induce plant resistance against pathogens, possibly through its volatile metabolites. Adequate culture media was inoculated, and its headspace was then sampled with a solid-phase microextraction fiber and chromatographed every 24 h over seven days. The raw chromatogram processing using multi-way principal component analysis allowed the determination of the inoculation period, during which the concentration of volatile metabolites was maximized, as well as the discrimination of the appropriate peaks from the complex culture media background. Several volatile metabolites not previously described in the literature on biocontrol fungi were observed, as well as sesquiterpenes and aliphatic alcohols. These results stress that, due to the complexity of multidimensional chromatographic data, multivariate tools might be mandatory even for apparently trivial tasks, such as the determination of the temporal profile of metabolite production and extinction. However, when compared with conventional gas chromatography, the complex data processing yields a considerable improvement in the information obtained from the samples. This article is protected by copyright. All rights reserved.
Subject: Comprehensive Two-dimensional Gas Chromatography
Metabolic Profile
Saprotroph Fungi
Solid-phase Microextraction
Citation: Journal Of Separation Science. , 2015-Mar.
Rights: fechado
Identifier DOI: 10.1002/jssc.201401404
Date Issue: 2015
Appears in Collections:Unicamp - Artigos e Outros Documentos

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