Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/202234
Type: Artigo de periódico
Title: Towards A Deeper Understanding Of Structural Biomass Recalcitrance Using Phase-contrast Tomography.
Author: Isaac, Augusta
Barboza, Vinicius
Sket, Federico Ivan
D'Almeida, José Roberto M
Montoro, Luciano Andrey
Hilger, André
Manke, Ingo
Abstract: The development of technological routes to convert lignocellulosic biomass to liquid fuels requires an in-depth understanding of the cell wall architecture of substrates. Essential pretreatment processes are conducted to reduce biomass recalcitrance and usually increase the reactive surface area. Quantitative three-dimensional information about both bulk and surface structural features of substrates needs to be obtained to expand our knowledge of substrates. In this work, phase-contrast tomography (PCT) was used to gather information about the structure of a model lignocellulosic biomass (piassava fibers). The three-dimensional cellular organization of piassava fibers was characterized by PCT using synchrotron radiation. This technique enabled important physical features that describe the substrate piassava fibers to be visualized and quantified. The external surface area of a fiber and internal surface area of the pores in a fiber could be determined separately. More than 96% of the overall surface area available to enzymes was in the bulk substrate. The pore surface area and length exhibited a positive linear relationship, where the slope of this relationship depended on the plant tissue. We demonstrated that PCT is a powerful tool for the three-dimensional characterization of the cell wall features related to biomass recalcitrance. Original and relevant quantitative information about the structural features of the analyzed material were obtained. The data obtained by PCT can be used to improve processing routes to efficiently convert biomass feedstock into sugars.
Subject: Biomass
Phase-contrast Tomography
Recalcitrance
Surface Area
Synchrotron Radiation
Rights: fechado
Identifier DOI: 10.1186/s13068-015-0229-8
Address: http://www.ncbi.nlm.nih.gov/pubmed/25788981
Date Issue: 2015
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

Files in This Item:
File SizeFormat 
pmed_25788981.pdf2.78 MBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.