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Type: Artigo de periódico
Title: G-quadruplex Formation Enhances Splicing Efficiency Of Pax9 Intron 1.
Author: Ribeiro, Mariana Martins
Teixeira, Gleidson Silva
Martins, Luciane
Marques, Marcelo Rocha
de Souza, Ana Paula
Line, Sergio Roberto Peres
Abstract: G-quadruplexes are secondary structures present in DNA and RNA molecules, which are formed by stacking of G-quartets (i.e., interaction of four guanines (G-tracts) bounded by Hoogsteen hydrogen bonding). Human PAX9 intron 1 has a putative G-quadruplex-forming region located near exon 1, which is present in all known sequenced placental mammals. Using circular dichroism (CD) analysis and CD melting, we showed that these sequences are able to form highly stable quadruplex structures. Due to the proximity of the quadruplex structure to exon-intron boundary, we used a validated double-reporter splicing assay and qPCR to analyze its role on splicing efficiency. The human quadruplex was shown to have a key role on splicing efficiency of PAX9 intron 1, as a mutation that abolished quadruplex formation decreased dramatically the splicing efficiency of human PAX9 intron 1. The less stable, rat quadruplex had a less efficient splicing when compared to human sequences. Additionally, the treatment with 360A, a strong ligand that stabilizes quadruplex structures, further increased splicing efficiency of human PAX9 intron 1. Altogether, these results provide evidences that G-quadruplex structures are involved in splicing efficiency of PAX9 intron 1.
Subject: Animals
Base Sequence
Circular Dichroism
Fluorescence Resonance Energy Transfer
Molecular Sequence Data
Pax9 Transcription Factor
Rna Splicing
Rna, Messenger
Reverse Transcriptase Polymerase Chain Reaction
Sequence Homology, Nucleic Acid
Rights: fechado
Identifier DOI: 10.1007/s00439-014-1485-6
Date Issue: 2015
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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