Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/202211
Type: Artigo de periódico
Title: Determination Of The Phenolic Composition From Brazilian Tropical Fruits By Uhplc-ms/ms.
Author: Bataglion, Giovana A
da Silva, Felipe M A
Eberlin, Marcos N
Koolen, Hector H F
Abstract: Although Brazil is the third largest fruit producer in the world, several specimens consumed are not well studied from the chemical viewpoint, especially for quantitative analysis. For this reason and the crescent employment of mass spectrometry (MS) techniques in food science we selected twenty-two phenolic compounds with important biological activities and developed an ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method using electrospray (ESI) in negative ion mode aiming their quantification in largely consumed Brazilian fruits (açaí-do-Amazonas, acerola, cashew apple, camu-camu, pineapple and taperebá). Multiple reaction monitoring (MRM) was applied and the selection of proper product ions for each transition assured high selectivity. Linearity (0.995<r(2)<0.999), limit of detection (28.85-333.3pg/mL), limit of quantification (96.15-1111pg/mL), inter- and intraday accuracy (>80%), precision (CV<20%) and extraction recovery rate (>80%) were satisfactory and showed that the method provides an efficient protocol to analyze phenolic compounds in fruit pulp extracts.
Subject: Flavonoids
Mass Spectrometry
Phenolic Acids
Quantification
Tropical Fruits
Rights: fechado
Identifier DOI: 10.1016/j.foodchem.2015.02.059
Address: http://www.ncbi.nlm.nih.gov/pubmed/25766829
Date Issue: 2015
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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