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Type: Artigo de periódico
Title: A Metabolomic Protocol For Plant Systematics By Matrix-assisted Laser-desorption/ionization Time-of Flight Mass Spectrometry.
Author: Ernst, Madeleine
Silva, Denise B
Silva, Ricardo
Monge, Marcelo
Semir, João
Vêncio, Ricardo Z N
Lopes, Norberto P
Abstract: Matrix-assisted laser desorption/ionization time-of flight mass spectrometry (MALDI-TOF MS) has been widely used for the identification and classification of microorganisms based on their proteomic fingerprints. However, the use of MALDI-TOF MS in plant research has been very limited. In the present study, a first protocol is proposed for metabolic fingerprinting by MALDI-TOF MS using three different MALDI matrices with subsequent multivariate data analysis by in-house algorithms implemented in the R environment for the taxonomic classification of plants from different genera, families and orders. By merging the data acquired with different matrices, different ionization modes and using careful algorithms and parameter selection, we demonstrate that a close taxonomic classification can be achieved based on plant metabolic fingerprints, with 92% similarity to the taxonomic classifications found in literature. The present work therefore highlights the great potential of applying MALDI-TOF MS for the taxonomic classification of plants and, furthermore, provides a preliminary foundation for future research.
Subject: Matrix-assisted Laser Desorption/ionization Time-of Flight Mass Spectrometry
Metabolic Fingerprinting
Plant Taxonomy
R Environment
Multivariate Data Analysis
Rights: fechado
Identifier DOI: 10.1016/j.aca.2015.01.002
Date Issue: 2015
Appears in Collections:Unicamp - Artigos e Outros Documentos

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