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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleAnkhd1 Silencing Inhibits Stathmin 1 Activity, Cell Proliferation And Migration Of Leukemia Cells.pt_BR
dc.contributor.authorMachado-Neto, João Agostinhopt_BR
dc.contributor.authorLazarini, Marianapt_BR
dc.contributor.authorFavaro, Patriciapt_BR
dc.contributor.authorde Melo Campos, Paulapt_BR
dc.contributor.authorScopim-Ribeiro, Renatapt_BR
dc.contributor.authorFranchi, Gilberto Carlospt_BR
dc.contributor.authorNowill, Alexandre Eduardopt_BR
dc.contributor.authorLima, Paulo Roberto Mourapt_BR
dc.contributor.authorCosta, Fernando Ferreirapt_BR
dc.contributor.authorBenichou, Sergept_BR
dc.contributor.authorOlalla Saad, Sara Teresinhapt_BR
dc.contributor.authorTraina, Fabiolapt_BR
unicamp.authorJoão Agostinho Machado-Neto, Hematology and Hemotherapy Center-University of Campinas/Hemocentro-Unicamp, Instituto Nacional de Ciência e Tecnologia do Sangue, Campinas 13083-878, São Paulo, Brazil.pt_BR
unicamp.authorMariana Lazarini, Hematology and Hemotherapy Center-University of Campinas/Hemocentro-Unicamp, Instituto Nacional de Ciência e Tecnologia do Sangue, Campinas 13083-878, São Paulo, Brazil.pt_BR
unicamp.authorPatricia Favaro, Hematology and Hemotherapy Center-University of Campinas/Hemocentro-Unicamp, Instituto Nacional de Ciência e Tecnologia do Sangue, Campinas 13083-878, São Paulo, Brazil.pt_BR
unicamp.authorPaula de Melo Campos, Hematology and Hemotherapy Center-University of Campinas/Hemocentro-Unicamp, Instituto Nacional de Ciência e Tecnologia do Sangue, Campinas 13083-878, São Paulo, Brazil.pt_BR
unicamp.authorRenata Scopim-Ribeiro, Hematology and Hemotherapy Center-University of Campinas/Hemocentro-Unicamp, Instituto Nacional de Ciência e Tecnologia do Sangue, Campinas 13083-878, São Paulo, Brazil.pt_BR
unicamp.authorGilberto Carlos Franchi, Integrated Center for Childhood Onco-Hematological Investigation, University of Campinas, Campinas 13083-878, São Paulo, Brazil.pt_BR
unicamp.authorAlexandre Eduardo Nowill, Integrated Center for Childhood Onco-Hematological Investigation, University of Campinas, Campinas 13083-878, São Paulo, Brazil.pt_BR
unicamp.authorPaulo Roberto Moura Lima, Hematology and Hemotherapy Center-University of Campinas/Hemocentro-Unicamp, Instituto Nacional de Ciência e Tecnologia do Sangue, Campinas 13083-878, São Paulo, Brazil.pt_BR
unicamp.authorFernando Ferreira Costa, Hematology and Hemotherapy Center-University of Campinas/Hemocentro-Unicamp, Instituto Nacional de Ciência e Tecnologia do Sangue, Campinas 13083-878, São Paulo, Brazil.pt_BR
unicamp.authorSara Teresinha Olalla Saad, Hematology and Hemotherapy Center-University of Campinas/Hemocentro-Unicamp, Instituto Nacional de Ciência e Tecnologia do Sangue, Campinas 13083-878, São Paulo, Brazil.pt_BR
unicamp.authorFabiola Traina, Hematology and Hemotherapy Center-University of Campinas/Hemocentro-Unicamp, Instituto Nacional de Ciência e Tecnologia do Sangue, Campinas 13083-878, São Paulo, Brazil. Electronic address: ftraina@fmrp.usp.br.pt_BR
unicamp.author.externalSerge Benichou, INSERM U1016, Institut Cochin, Paris, France.pt
dc.subjectAnkhd1pt_BR
dc.subjectAcute Leukemiapt_BR
dc.subjectCell Proliferationpt_BR
dc.subjectSiva1pt_BR
dc.subjectStathmin 1pt_BR
dc.description.abstractANKHD1 is highly expressed in human acute leukemia cells and potentially regulates multiple cellular functions through its ankyrin-repeat domains. In order to identify interaction partners of the ANKHD1 protein and its role in leukemia cells, we performed a yeast two-hybrid system screen and identified SIVA, a cellular protein known to be involved in proapoptotic signaling pathways. The interaction between ANKHD1 and SIVA was confirmed by co-imunoprecipitation assays. Using human leukemia cell models and lentivirus-mediated shRNA approaches, we showed that ANKHD1 and SIVA proteins have opposing effects. While it is known that SIVA silencing promotes Stathmin 1 activation, increased cell migration and xenograft tumor growth, we showed that ANKHD1 silencing leads to Stathmin 1 inactivation, reduced cell migration and xenograft tumor growth, likely through the inhibition of SIVA/Stathmin 1 association. In addition, we observed that ANKHD1 knockdown decreases cell proliferation, without modulating apoptosis of leukemia cells, while SIVA has a proapoptotic function in U937 cells, but does not modulate proliferation in vitro. Results indicate that ANKHD1 binds to SIVA and has an important role in inducing leukemia cell proliferation and migration via the Stathmin 1 pathway. ANKHD1 may be an oncogene and participate in the leukemia cell phenotype.en
dc.relation.ispartofBiochimica Et Biophysica Actapt_BR
dc.relation.ispartofabbreviationBiochim. Biophys. Actapt_BR
dc.date.issued2015-Marpt_BR
dc.identifier.citationBiochimica Et Biophysica Acta. v. 1853, n. 3, p. 583-593, 2015-Mar.pt_BR
dc.language.isoengpt_BR
dc.description.volume1853pt_BR
dc.description.firstpage583-593pt_BR
dc.rightsfechadopt_BR
dc.rights.holderCopyright © 2014 Elsevier B.V. All rights reserved.pt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn0006-3002pt_BR
dc.identifier.doi10.1016/j.bbamcr.2014.12.012pt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/25523139pt_BR
dc.date.available2015-11-27T13:45:47Z-
dc.date.accessioned2015-11-27T13:45:47Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T13:45:47Z (GMT). No. of bitstreams: 1 pmed_25523139.pdf: 2237471 bytes, checksum: 3ded258d969e6c3b3f4cf35017366c7f (MD5) Previous issue date: 2015en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/202005-
dc.identifier.idPubmed25523139pt_BR
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