Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/201958
Type: Artigo de periódico
Title: Tissue Microarray Is A Reliable Method For Immunohistochemical Analysis Of Pleomorphic Adenoma.
Author: Fonseca, Felipe Paiva
de Andrade, Bruno Augusto Benevenuto
Rangel, Ana Lúcia Carrinho Ayrosa
Della Coletta, Ricardo
Lopes, Márcio Ajudarte
de Almeida, Oslei Paes
Vargas, Pablo Agustin
Abstract: To determine the most adequate number and size of tissue microarray (TMA) cores for pleomorphic adenoma immunohistochemical studies. Eighty-two pleomorphic adenoma cases were distributed in 3 TMA blocks assembled in triplicate containing 1.0-, 2.0-, and 3.0-mm cores. Immunohistochemical analysis against cytokeratin 7, Ki67, p63, and CD34 were performed and subsequently evaluated with PixelCount, nuclear, and microvessel software applications. The 1.0-mm TMA presented lower results than 2.0- and 3.0-mm TMAs versus conventional whole section slides. Possibly because of an increased amount of stromal tissue, 3.0-mm cores presented a higher microvessel density. Comparing the results obtained with one, two, and three 2.0-mm cores, there was no difference between triplicate or duplicate TMAs and a single-core TMA. Considering the possible loss of cylinders during immunohistochemical reactions, 2.0-mm TMAs in duplicate are a more reliable approach for pleomorphic adenoma immunohistochemical study.
Subject: Adenoma, Pleomorphic
Adolescent
Adult
Aged
Aged, 80 And Over
Child
Female
Humans
Image Interpretation, Computer-assisted
Immunohistochemistry
Male
Middle Aged
Reproducibility Of Results
Salivary Gland Neoplasms
Tissue Array Analysis
Tumor Markers, Biological
Rights: fechado
Identifier DOI: 10.1016/j.oooo.2013.08.029
Address: http://www.ncbi.nlm.nih.gov/pubmed/24332331
Date Issue: 2014
Appears in Collections:Unicamp - Artigos e Outros Documentos

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