Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/201930
Type: Artigo de periódico
Title: Clustering Of Water Bodies In Unpolluted And Polluted Environments Based On Escherichia Coli Phylogroup Abundance Using A Simple Interaction Database.
Author: de Castro Stoppe, Nancy
Silva, Juliana Saragiotto
Torres, Tatiana Teixeira
Carlos, Camila
Hachich, Elayse Maria
Sato, Maria Inês Zanoli
Saraiva, Antonio Mauro
Ottoboni, Laura Maria Mariscal
Abstract: Different types of water bodies, including lakes, streams, and coastal marine waters, are often susceptible to fecal contamination from a range of point and nonpoint sources, and have been evaluated using fecal indicator microorganisms. The most commonly used fecal indicator is Escherichia coli, but traditional cultivation methods do not allow discrimination of the source of pollution. The use of triplex PCR offers an approach that is fast and inexpensive, and here enabled the identification of phylogroups. The phylogenetic distribution of E. coli subgroups isolated from water samples revealed higher frequencies of subgroups A1 and B23 in rivers impacted by human pollution sources, while subgroups D1 and D2 were associated with pristine sites, and subgroup B1 with domesticated animal sources, suggesting their use as a first screening for pollution source identification. A simple classification is also proposed based on phylogenetic subgroup distribution using the w-clique metric, enabling differentiation of polluted and unpolluted sites.
Subject: E. Coli
Interaction Networks
Phylogenetic Groups
Pollution Sources
Social Network Analysis
Rights: aberto
Identifier DOI: 10.1590/S1415-47572014005000016
Address: http://www.ncbi.nlm.nih.gov/pubmed/25505844
Date Issue: 2014
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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