Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/201929
Type: Artigo de periódico
Title: Analysis Of The Ergosterol Biosynthesis Pathway Cloning, Molecular Characterization And Phylogeny Of Lanosterol 14 α-demethylase (erg11) Gene Of Moniliophthora Perniciosa.
Author: de Oliveira Ceita, Geruza
Vilas-Boas, Laurival Antônio
Castilho, Marcelo Santos
Carazzolle, Marcelo Falsarella
Pirovani, Carlos Priminho
Selbach-Schnadelbach, Alessandra
Gramacho, Karina Peres
Ramos, Pablo Ivan Pereira
Barbosa, Luciana Veiga
Pereira, Gonçalo Amarante Guimarães
Góes-Neto, Aristóteles
Abstract: The phytopathogenic fungus Moniliophthora perniciosa (Stahel) Aime & Philips-Mora, causal agent of witches' broom disease of cocoa, causes countless damage to cocoa production in Brazil. Molecular studies have attempted to identify genes that play important roles in fungal survival and virulence. In this study, sequences deposited in the M. perniciosa Genome Sequencing Project database were analyzed to identify potential biological targets. For the first time, the ergosterol biosynthetic pathway in M. perniciosa was studied and the lanosterol 14α-demethylase gene (ERG11) that encodes the main enzyme of this pathway and is a target for fungicides was cloned, characterized molecularly and its phylogeny analyzed. ERG11 genomic DNA and cDNA were characterized and sequence analysis of the ERG11 protein identified highly conserved domains typical of this enzyme, such as SRS1, SRS4, EXXR and the heme-binding region (HBR). Comparison of the protein sequences and phylogenetic analysis revealed that the M. perniciosa enzyme was most closely related to that of Coprinopsis cinerea.
Subject: Basidiomycota
Theobroma Cacao
Ergosterol
Fungus
Rights: aberto
Identifier DOI: 10.1590/S1415-47572014005000017
Address: http://www.ncbi.nlm.nih.gov/pubmed/25505843
Date Issue: 2014
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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