Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/201888
Type: Artigo de periódico
Title: Development And Characterization Of A Cationic Lipid Nanocarrier As Non-viral Vector For Gene Therapy.
Author: Severino, Patrícia
Szymanski, Marcelo
Favaro, Marianna
Azzoni, Adriano R
Chaud, Marco V
Santana, Maria Helena A
Silva, Amélia M
Souto, Eliana B
Abstract: The aim of the present work was to produce a cationic solid lipid nanoparticle (SLN) as non-viral vector for protein delivery. Cationic SLN were produced by double emulsion method, composed of softisan(®) 100, cetyltrimethylammonium bromide (CTAB), Tween(®) 80, Span(®) 80, glycerol and lipoid(®) S75 loading insulin as model protein. The formulation was characterized in terms of mean hydrodynamic diameter (z-ave), polydispersity index (PI), zeta potential (ZP), stability during storage time, stability after lyophilization, effect of toxicity and transfection ability in HeLa cells, in vitro release profile and morphology. SLN were stable for 30days and showed minimal changes in their physicochemical properties after lyophilization. The particles exhibited a relatively slow release, spherical morphology and were able to transfect HeLa cells, but toxicity remained an obstacle. Results suggest that SLN are nevertheless promising for delivery of proteins or nucleic acids for gene therapy.
Subject: Gene Delivery
Nanotechnology
Non-viral Vectors
Proteins
Solid Lipid Nanoparticles
Rights: fechado
Identifier DOI: 10.1016/j.ejps.2014.09.021
Address: http://www.ncbi.nlm.nih.gov/pubmed/25446510
Date Issue: 2014
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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