Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/201827
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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleLong-term Spinal Ventral Root Reimplantation, But Not Bone Marrow Mononuclear Cell Treatment, Positively Influences Ultrastructural Synapse Recovery And Motor Axonal Regrowth.pt_BR
dc.contributor.authorBarbizan, Robertapt_BR
dc.contributor.authorCastro, Mateus Vpt_BR
dc.contributor.authorFerreira, Rui Seabrapt_BR
dc.contributor.authorBarraviera, Beneditopt_BR
dc.contributor.authorOliveira, Alexandre L Rpt_BR
unicamp.authorRoberta Barbizan, Department of Structural and Functional Biology, University of Campinas (UNICAMP), PO Box 6109, Campinas 13083-970, São Paulo, Brazil. robertabarbizan@yahoo.com.br.pt_BR
unicamp.authorMateus V Castro, Department of Structural and Functional Biology, University of Campinas (UNICAMP), PO Box 6109, Campinas 13083-970, São Paulo, Brazil. mateusvidigal@hotmail.com.pt_BR
unicamp.authorAlexandre L R Oliveira, Department of Structural and Functional Biology, University of Campinas (UNICAMP), PO Box 6109, Campinas 13083-970, São Paulo, Brazil. alroliv@unicamp.br.pt_BR
unicamp.author.externalRui Seabra Ferreira, Center for the Study of Venoms and Venomous Animals (CEVAP), São Paulo State University (UNESP), Botucatu 18610-307, São Paulo, Brazil. rui.ead@gmail.com.pt
unicamp.author.externalBenedito Barraviera, Center for the Study of Venoms and Venomous Animals (CEVAP), São Paulo State University (UNESP), Botucatu 18610-307, São Paulo, Brazil. bbviera@gnosis.com.br.pt
dc.description.abstractWe recently proposed a new surgical approach to treat ventral root avulsion, resulting in motoneuron protection. The present work combined such a surgical approach with bone marrow mononuclear cells (MC) therapy. Therefore, MC were added to the site of reimplantation. Female Lewis rats (seven weeks old) were subjected to unilateral ventral root avulsion (VRA) at L4, L5 and L6 levels and divided into the following groups (n = 5 for each group): Avulsion, sealant reimplanted roots and sealant reimplanted roots plus MC. After four weeks and 12 weeks post-surgery, the lumbar intumescences were processed by transmission electron microscopy, to analyze synaptic inputs to the repaired α motoneurons. Also, the ipsi and contralateral sciatic nerves were processed for axon counting and morphometry. The ultrastructural results indicated a significant preservation of inhibitory pre-synaptic boutons in the groups repaired with sealant alone and associated with MC therapy. Moreover, the average number of axons was higher in treated groups when compared to avulsion only. Complementary to the fiber counting, the morphometric analysis of axonal diameter and g ratio demonstrated that root reimplantation improved the motor component recovery. In conclusion, the data herein demonstrate that root reimplantation at the lesion site may be considered a therapeutic approach, following proximal lesions in the interface of central nervous system (CNS) and peripheral nervous system (PNS), and that MC therapy does not further improve the regenerative recovery, up to 12 weeks post lesion.en
dc.relation.ispartofInternational Journal Of Molecular Sciencespt_BR
dc.relation.ispartofabbreviationInt J Mol Scipt_BR
dc.date.issued2014pt_BR
dc.identifier.citationInternational Journal Of Molecular Sciences. v. 15, n. 11, p. 19535-51, 2014.pt_BR
dc.language.isoengpt_BR
dc.description.volume15pt_BR
dc.description.initialpage19535-51pt_BR
dc.rightsabertopt_BR
dc.rights.holderpt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn1422-0067pt_BR
dc.identifier.doi10.3390/ijms151119535pt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/25353176pt_BR
dc.date.available2015-11-27T13:43:40Z-
dc.date.accessioned2015-11-27T13:43:40Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T13:43:40Z (GMT). No. of bitstreams: 1 pmed_25353176.pdf: 2726858 bytes, checksum: a5c23f601e6519cc077deeea46c1a4b6 (MD5) Previous issue date: 2014en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/201827-
dc.identifier.idPubmed25353176pt_BR
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