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Type: Artigo de periódico
Title: Mesenchymal Stromal Cells From Adipose Tissue Attached To Suture Material Enhance The Closure Of Enterocutaneous Fistulas In A Rat Model.
Author: Volpe, Bruno Bosch
Santos Duarte, Adriana da Silva
Ribeiro, Thiago Borsoi
Stocchero, Ithamar
Kharmandayan, Paulo
Olalla Saad, Sara Teresinha
Bustorff-Silva, Joaquim Murray
Malheiros Luzo, Angela Cristina
Abstract: Surgical treatment for enterocutaneous fistulas (EF) frequently fails. Cell therapy may represent a new approach to treatment. Mesenchymal stromal cells (MSCs) have high proliferative and differentiation capacity. This study aimed to investigate whether MSCs could adhere to suture filament (SF), promoting better EF healing. MSCs, 1 × 10(6), from adipose tissue (ATMSCs) were adhered to a Polyvicryl SF by adding a specific fibrin glue formulation. Adhesion was confirmed by confocal and scanning electron microscopy (SEM). A cecal fistula was created in 22 Wistar rats by incising the cecum and suturing the opening to the surgical wound subcutaneously with four separate stitches. The animals were randomly allocated to three groups: control (CG)-five animals, EF performed; injection (IG)-eight animals 1 × 10(6) ATMSCs injected around EF borders; and suture filament (SG): nine animals, sutured with 1 × 10(6) ATMSCs attached to the filaments with fibrin glue. Fistulas were photographed on the operation day and every 3 days until the 21st day and analyzed by two observers using ImageJ Software. Confocal and SEM results demonstrated ATMSCs adhered to SF (ATMSCs-SF). The average reduction size of the fistula area at 21st day was greater for the SG group (90.34%, P < 0.05) than the IG (71.80%) and CG (46.54%) groups. ATMSCs adhered to SF maintain viability and proliferative capacity. EF submitted to ATMSCs-SF procedure showed greater recovery and healing. This approach might be a new and effective tool for EF treatment.
Subject: Crohn Disease
Cell Therapy
Enterocutaneous Fistula
Mesenchymal Stem Cells
Suture Filaments
Rights: fechado
Identifier DOI: 10.1016/j.jcyt.2014.08.004
Date Issue: 2014
Appears in Collections:Unicamp - Artigos e Outros Documentos

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