Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/201793
Type: Artigo de periódico
Title: Anxiety-like Effects Of Meta-chlorophenylpiperazine In Paradoxically Sleep-deprived Mice.
Author: Polesel, Daniel Ninello
Fukushiro, Daniela Fukue
Andersen, Monica Levy
Nozoe, Karen Tieme
Mári-Kawamoto, Elisa
Saito, Luís Paulo
Carvalho, Fábio Ramos Souza
Alvarenga, Tathiana Aparecida
Freitas, Denise
Tufik, Sergio
Frussa-Filho, Roberto
Lanaro, Rafael
Costa, José Luiz
Tavares, Marina Franco Maggi
Abstract: Chlorophenylpiperazines (CPP) are psychotropic drugs used in nightclub parties and are frequently used in a state of sleep deprivation, a condition which can potentiate the effects of psychoactive drugs. This study aimed to investigate the effects of sleep deprivation and sleep rebound (RB) on anxiety-like measures in mCPP-treated mice using the open field test. We first optimized our procedure by performing dose-effect curves and examining different pretreatment times in naïve male Swiss mice. Subsequently, a separate cohort of mice underwent paradoxical sleep deprivation (PSD) for 24 or 48h. In the last experiment, immediately after the 24h-PSD period, mice received an injection of saline or mCPP, but their general activity was quantified in the open field only after the RB period (24 or 48h). The dose of 5mgmL(-1) of mCPP was the most effective at decreasing rearing behavior, with peak effects 15min after injection. PSD decreased locomotion and rearing behaviors, thereby inhibiting a further impairment induced by mCPP. Plasma concentrations of mCPP were significantly higher in PSD 48h animals compared to the non-PSD control group. Twenty-four hours of RB combined with mCPP administration produced a slight reduction in locomotion. Our results show that mCPP was able to significantly change the behavior of naïve, PSD, and RB mice. When combined with sleep deprivation, there was a higher availability of drug in plasma levels. Taken together, our results suggest that sleep loss can enhance the behavioral effects of the potent psychoactive drug, mCPP, even after a period of rebound sleep.
Subject: Animals
Anxiety
Behavior, Animal
Designer Drugs
Dose-response Relationship, Drug
Male
Mice
Piperazines
Sleep Deprivation
Cpp
Ctrl
Chlorophenylpiperazines
Control
Designer Drugs
Intraperitoneal
Lc-ms/ms
Liquid Chromatography-tandem Mass Spectrometry
Mdma
Mtbe
Meta-chlorophenylpiperazine
Methyl Tert-butyl Ether
Methylenedioxymethamphetamine
Of
Open Field
Orto-chlorophenylpiperazine
Psd
Paradoxical Sleep Deprivation
Qc
Quality Control
Rb
Srm
Selected Reaction Monitoring
Sleep Deprivation
Sleep Rebound
Ip
Mcpp
Ocpp
Rights: fechado
Identifier DOI: 10.1016/j.pnpbp.2013.11.007
Address: http://www.ncbi.nlm.nih.gov/pubmed/24269663
Date Issue: 2014
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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