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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleEffects Of Sterilization Methods On The Physical, Chemical, And Biological Properties Of Silk Fibroin Membranes.pt_BR
dc.contributor.authorde Moraes, Mariana Agostinipt_BR
dc.contributor.authorWeska, Raquel Fariaspt_BR
dc.contributor.authorBeppu, Marisa Masumipt_BR
unicamp.authorMariana Agostini de Moraes, School of Chemical Engineering, University of Campinas, UNICAMP, 13083-852, Campinas, SP, Brazil.pt_BR
unicamp.author.externalRaquel Farias Weska,pt
unicamp.author.externalMarisa Masumi Beppu,pt
dc.subjectAnimalspt_BR
dc.subjectBombyxpt_BR
dc.subjectCho Cellspt_BR
dc.subjectCricetinaept_BR
dc.subjectCricetuluspt_BR
dc.subjectEthanolpt_BR
dc.subjectEthylene Oxidept_BR
dc.subjectFibroinspt_BR
dc.subjectGamma Rayspt_BR
dc.subjectHot Temperaturept_BR
dc.subjectHumiditypt_BR
dc.subjectMaterials Testingpt_BR
dc.subjectMembranes, Artificialpt_BR
dc.subjectPorositypt_BR
dc.subjectSilkpt_BR
dc.subjectSpectroscopy, Fourier Transform Infraredpt_BR
dc.subjectSterilizationpt_BR
dc.subjectTemperaturept_BR
dc.subjectTensile Strengthpt_BR
dc.subjectUltraviolet Rayspt_BR
dc.subjectX-ray Diffractionpt_BR
dc.subjectBiomaterialspt_BR
dc.subjectDense Membranespt_BR
dc.subjectFibroinpt_BR
dc.subjectPorous Membranespt_BR
dc.subjectSterilizationpt_BR
dc.description.abstractSilk fibroin has been widely explored for many biomedical applications, due to its biocompatibility and biodegradability. Sterilization is a fundamental step in biomaterials processing and it must not jeopardize the functionality of medical devices. The aim of this study was to analyze the influence of different sterilization methods in the physical, chemical, and biological characteristics of dense and porous silk fibroin membranes. Silk fibroin membranes were treated by several procedures: immersion in 70% ethanol solution, ultraviolet radiation, autoclave, ethylene oxide, and gamma radiation, and were analyzed by scanning electron microscopy, Fourier-transformed infrared spectroscopy (FTIR), X-ray diffraction, tensile strength and in vitro cytotoxicity to Chinese hamster ovary cells. The results indicated that the sterilization methods did not cause perceivable morphological changes in the membranes and the membranes were not toxic to cells. The sterilization methods that used organic solvent or an increased humidity and/or temperature (70% ethanol, autoclave, and ethylene oxide) increased the silk II content in the membranes: the dense membranes became more brittle, while the porous membranes showed increased strength at break. Membranes that underwent sterilization by UV and gamma radiation presented properties similar to the nonsterilized membranes, mainly for tensile strength and FTIR results.en
dc.relation.ispartofJournal Of Biomedical Materials Research. Part B, Applied Biomaterialspt_BR
dc.relation.ispartofabbreviationJ. Biomed. Mater. Res. Part B Appl. Biomater.pt_BR
dc.date.issued2014-Maypt_BR
dc.identifier.citationJournal Of Biomedical Materials Research. Part B, Applied Biomaterials. v. 102, n. 4, p. 869-76, 2014-May.pt_BR
dc.language.isoengpt_BR
dc.description.volume102pt_BR
dc.description.firstpage869-76pt_BR
dc.rightsfechadopt_BR
dc.rights.holderCopyright © 2013 Wiley Periodicals, Inc.pt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn1552-4981pt_BR
dc.identifier.doi10.1002/jbm.b.33069pt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/24259492pt_BR
dc.date.available2015-11-27T13:43:26Z-
dc.date.accessioned2015-11-27T13:43:26Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T13:43:26Z (GMT). No. of bitstreams: 1 pmed_24259492.pdf: 599761 bytes, checksum: c2cc9dd478520e927402852cbf23443b (MD5) Previous issue date: 2014en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/201749-
dc.identifier.idPubmed24259492pt_BR
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