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Type: Artigo de periódico
Title: Platinum Blue Staining Of Cells Grown In Electrospun Scaffolds.
Author: Yusuf, Mohammed
Millas, Ana Luiza G
Estandarte, Ana Katrina C
Bhella, Gurdeep K
McKean, Robert
Bittencourt, Edison
Robinson, Ian K
Abstract: Fibroblast cells grown in electrospun polymer scaffolds were stained with platinum blue, a heavy metal stain, and imaged using scanning electron microscopy. Good contrast on the cells was achieved compared with samples that were gold sputter coated. The cell morphology could be clearly observed, and the cells could be distinguished from the scaffold fibers. Here we optimized the required concentration of platinum blue for imaging cells grown in scaffolds and show that a higher concentration causes platinum aggregation. Overall, platinum blue is a useful stain for imaging cells because of its enhanced contrast using scanning electron microscopy (SEM). In the future it would be useful to investigate cell growth and morphology using three-dimensional imaging methods.
Subject: Cells
Platinum Blue
Scanning Electron Microscopy
Sputter Coating
Citation: Biotechniques. v. 57, n. 3, p. 137-41, 2014.
Rights: fechado
Identifier DOI: 10.2144/000114206
Date Issue: 2014
Appears in Collections:Unicamp - Artigos e Outros Documentos

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