Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/201704
Type: Artigo de periódico
Title: Development And Characterization Of 32 Microsatellite Loci In Genipa Americana (rubiaceae).
Author: Manoel, Ricardo O
Freitas, Miguel L M
Barreto, Mariana A
Moraes, Mário L T
Souza, Anete P
Sebbenn, Alexandre M
Abstract: • Microsatellite primers were developed for the tree species Genipa americana (Rubiaceae) for further population genetic studies. • We identified 144 clones containing 65 repeat motifs from a genomic library enriched for (CT)8 and (GT)8 motifs. Primer pairs were developed for 32 microsatellite loci and validated in 40 individuals of two natural G. americana populations. Seventeen loci were polymorphic, revealing from three to seven alleles per locus. The observed and expected heterozygosities ranged from 0.24 to 1.00 and from 0.22 to 0.78, respectively. • The 17 primers identified as polymorphic loci are suitable to study the genetic diversity and structure, mating system, and gene flow in G. americana.
Subject: Genipa Americana
Rubiaceae
Conservation Genetics
Microsatellite Markers
Population Genetics
Tree Species
Rights: fechado
Identifier DOI: 10.3732/apps.1300084
Address: http://www.ncbi.nlm.nih.gov/pubmed/25202610
Date Issue: 2014
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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