Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/201663
Type: Artigo de periódico
Title: A Modified Acidic Approach For Dna Extraction From Plant Species Containing High Levels Of Secondary Metabolites.
Author: Cavallari, M M
Siqueira, M V B M
Val, T M
Pavanelli, J C
Monteiro, M
Grando, C
Pinheiro, J B
Zucchi, M I
Gimenes, M A
Abstract: Purified genomic DNA can be difficult to obtain from some plant species because of the presence of impurities such as polysaccharides, which are often co-extracted with DNA. In this study, we developed a fast, simple, and low-cost protocol for extracting DNA from plants containing high levels of secondary metabolites. This protocol does not require the use of volatile toxic reagents such as mercaptoethanol, chloroform, or phenol and allows the extraction of high-quality DNA from wild and cultivated tropical species.
Rights: aberto
Identifier DOI: 10.4238/2014.August.25.13
Address: http://www.ncbi.nlm.nih.gov/pubmed/25158268
Date Issue: 2014
Appears in Collections:Unicamp - Artigos e Outros Documentos

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