Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/201639
Type: Artigo de periódico
Title: Bile And Liver Metallothionein Behavior In Copper-exposed Fish.
Author: Hauser-Davis, Rachel A
Bastos, Frederico F
Tuton, Bernardo
Chávez Rocha, Rafael
Saint' Pierre, Tatiana
Ziolli, Roberta L
Arruda, Marco A Z
Abstract: The present study analyzed metallothionein (MT) excretion from liver to bile in Nile Tilapia (Oreochromis niloticus) exposed to sub-lethal copper concentrations (2mgL(-1)) in a laboratory setting. MTs in liver and bile were quantified by spectrophotometry after thermal incubation and MT metal-binding profiles were characterized by size exclusion high performance liquid chromatography coupled to ICP-MS (SEC-HPLC-ICP-MS). Results show that liver MT is present in approximately 250-fold higher concentrations than bile MT in non-exposed fish. Differences between the MT profiles from the control and exposed group were observed for both matrices, indicating differential metal-binding behavior when comparing liver and bile MT. This is novel data regarding intra-organ MT comparisons, since differences between organs are usually present only with regard to quantification, not metal-binding behavior. Bile MT showed statistically significant differences between the control and exposed group, while the same did not occur with liver MT. This indicates that MTs synthesized in the liver accumulate more slowly than MTs excreted from liver to bile, since the same fish presented significantly higher MT levels in liver when compared to bile. We postulate that bile, although excreted in the intestine and partially reabsorbed by the same returning to the liver, may also release MT-bound metals more rapidly and efficiently, which may indicate an efficient detoxification route. Thus, we propose that the analysis of bile MTs to observe recent metal exposure may be more adequate than the analysis of liver MTs, since organism responses to metals are more quickly observed in bile, although further studies are necessary.
Subject: Animals
Bile
Copper
Electrophoresis, Polyacrylamide Gel
Liver
Metallothionein
Bile
Cu Contamination
Fish
Liver
Metallothionein
Rights: fechado
Identifier DOI: 10.1016/j.jtemb.2013.09.003
Address: http://www.ncbi.nlm.nih.gov/pubmed/24210855
Date Issue: 2014
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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