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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleReduced Insulin Clearance And Lower Insulin-degrading Enzyme Expression In The Liver Might Contribute To The Thrifty Phenotype Of Protein-restricted Mice.pt_BR
dc.contributor.authorRezende, Luiz Fpt_BR
dc.contributor.authorCamargo, Rafael Lpt_BR
dc.contributor.authorBranco, Renato C Spt_BR
dc.contributor.authorCappelli, Ana P Gpt_BR
dc.contributor.authorBoschero, Antonio Cpt_BR
dc.contributor.authorCarneiro, Everardo Mpt_BR
unicamp.authorLuiz F Rezende, Department of Structural and Functional Biology,Institute of Biology, State University of Campinas (UNICAMP),PO Box 6109,Campinas,SP,CEP 13083-865,Brazil.pt_BR
unicamp.authorRafael L Camargo, Department of Structural and Functional Biology,Institute of Biology, State University of Campinas (UNICAMP),PO Box 6109,Campinas,SP,CEP 13083-865,Brazil.pt_BR
unicamp.authorRenato C S Branco, Department of Structural and Functional Biology,Institute of Biology, State University of Campinas (UNICAMP),PO Box 6109,Campinas,SP,CEP 13083-865,Brazil.pt_BR
unicamp.authorAna P G Cappelli, Department of Structural and Functional Biology,Institute of Biology, State University of Campinas (UNICAMP),PO Box 6109,Campinas,SP,CEP 13083-865,Brazil.pt_BR
unicamp.authorAntonio C Boschero, Department of Structural and Functional Biology,Institute of Biology, State University of Campinas (UNICAMP),PO Box 6109,Campinas,SP,CEP 13083-865,Brazil.pt_BR
unicamp.authorEverardo M Carneiro, Department of Structural and Functional Biology,Institute of Biology, State University of Campinas (UNICAMP),PO Box 6109,Campinas,SP,CEP 13083-865,Brazil.pt_BR
dc.subjectAdipose Tissue, Whitept_BR
dc.subjectAnimalspt_BR
dc.subjectDiet, Protein-restrictedpt_BR
dc.subjectDown-regulationpt_BR
dc.subjectEnergy Intakept_BR
dc.subjectEnergy Metabolismpt_BR
dc.subjectGene Expression Regulation, Enzymologicpt_BR
dc.subjectInsulinpt_BR
dc.subjectInsulin Resistancept_BR
dc.subjectInsulysinpt_BR
dc.subjectIslets Of Langerhanspt_BR
dc.subjectLiverpt_BR
dc.subjectMicept_BR
dc.subjectMice, Inbred C57blpt_BR
dc.subjectMuscle, Skeletalpt_BR
dc.subjectPhosphorylationpt_BR
dc.subjectProtein Processing, Post-translationalpt_BR
dc.subjectRandom Allocationpt_BR
dc.subjectReceptor, Insulinpt_BR
dc.subjectSignal Transductionpt_BR
dc.subjectWeaningpt_BR
dc.subjectWeight Gainpt_BR
dc.description.abstractNutrient restriction during the early stages of life usually leads to alterations in glucose homeostasis, mainly insulin secretion and sensitivity, increasing the risk of metabolic disorders in adulthood. Despite growing evidence regarding the importance of insulin clearance during glucose homeostasis in health and disease, no information exists about this process in malnourished animals. Thus, in the present study, we aimed to determine the effect of a nutrient-restricted diet on insulin clearance using a model in which 30-d-old C57BL/6 mice were exposed to a protein-restricted diet for 14 weeks. After this period, we evaluated many metabolic variables and extracted pancreatic islet, liver, gastrocnemius muscle (GCK) and white adipose tissue samples from the control (normal-protein diet) and restricted (low-protein diet, LP) mice. Insulin concentrations were determined using RIA and protein expression and phosphorylation by Western blot analysis. The LP mice exhibited lower body weight, glycaemia, and insulinaemia, increased glucose tolerance and altered insulin dynamics after the glucose challenge. The improved glucose tolerance could partially be explained by an increase in insulin sensitivity through the phosphorylation of the insulin receptor/protein kinase B and AMP-activated protein kinase/acetyl-CoA carboxylase in the liver, whereas the changes in insulin dynamics could be attributed to reduced insulin secretion coupled with reduced insulin clearance and lower insulin-degrading enzyme (IDE) expression in the liver and GCK. In summary, protein-restricted mice not only produce and secrete less insulin, but also remove and degrade less insulin. This phenomenon has the double benefit of sparing insulin while prolonging and potentiating its effects, probably due to the lower expression of IDE in the liver, possibly with long-term consequences.en
dc.relation.ispartofThe British Journal Of Nutritionpt_BR
dc.relation.ispartofabbreviationBr. J. Nutr.pt_BR
dc.date.issued2014-Seppt_BR
dc.identifier.citationThe British Journal Of Nutrition. v. 112, n. 6, p. 900-7, 2014-Sep.pt_BR
dc.language.isoengpt_BR
dc.description.volume112pt_BR
dc.description.firstpage900-7pt_BR
dc.rightsfechadopt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn1475-2662pt_BR
dc.identifier.doi10.1017/S0007114514001238pt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/25036874pt_BR
dc.date.available2015-11-27T13:42:55Z-
dc.date.accessioned2015-11-27T13:42:55Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T13:42:55Z (GMT). No. of bitstreams: 1 pmed_25036874.pdf: 349118 bytes, checksum: 0992bb0130b99d241bd13b13095a3aec (MD5) Previous issue date: 2014en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/201555-
dc.identifier.idPubmed25036874pt_BR
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