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Type: Artigo de periódico
Title: Rat Atrial Responses To Bothrops Jararacussu (jararacuçu) Snake Venom.
Author: Rodrigues, Mariana A P
Dias, Lourdes
Rennó, André L
Sousa, Norma C
Smaal, Adriana
da Silva, Delano A
Hyslop, Stephen
Abstract: Envenoming by the pitviper Bothrops jararacussu produces cardiovascular alterations, including coagulopathy, systemic hemorrhage, hypotension, circulatory shock and renal failure. In this work, we examined the activity of this venom in rat isolated right atria. Incubation with venom (0.025, 0.05, 0.1 and 0.2mg/ml) caused concentration-dependent muscle contracture that was not reversed by washing. Muscle damage was seen histologically and confirmed by quantification of creatine kinase-MB (CK-MB) release. Heating and preincubation of venom with p-bromophenacyl bromide (a phospholipase A2 inhibitor) abolished the venom-induced contracture and muscle damage. In contrast, indomethacin, a non-selective inhibitor of cyclooxygenase, and verapamil, a voltage-gated Ca(2+) channel blocker, did not affect the responses to venom. Preincubation of venom with Bothrops or Bothrops/Crotalus antivenom or the addition of antivenom soon after venom attenuated the venom-induced changes in atrial function and tissue damage. These results indicate that B. jararacussu venom adversely affected rat atrial contractile activity and muscle organization through the action of venom PLA2; these venom-induced alterations were attenuated by antivenom.
Subject: Acetophenones
Calcium Channel Blockers
Creatine Kinase, Mb Form
Crotalid Venoms
Heart Atria
In Vitro Techniques
Myocardial Contraction
Phospholipase A2 Inhibitors
Phospholipases A2
Rats, Wistar
Bothrops Jararacussu Venom
Muscle Contracture
Phospholipase A(2)
Rat Atria
Rights: fechado
Identifier DOI: 10.1016/j.tox.2014.06.010
Date Issue: 2014
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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