Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/201499
Type: Artigo de periódico
Title: Metabolic Memory Of ß-cells Controls Insulin Secretion And Is Mediated By Camkii.
Author: Santos, Gustavo Jorge Dos
Ferreira, Sandra Mara
Ortis, Fernanda
Rezende, Luiz Fernando
Li, Chengyang
Naji, Ali
Carneiro, Everardo Magalhães
Kaestner, Klaus H
Boschero, Antonio Carlos
Abstract: Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) functions both in regulation of insulin secretion and neurotransmitter release through common downstream mediators. Therefore, we hypothesized that pancreatic ß-cells acquire and store the information contained in calcium pulses as a form of metabolic memory, just as neurons store cognitive information. To test this hypothesis, we developed a novel paradigm of pulsed exposure of ß-cells to intervals of high glucose, followed by a 24-h consolidation period to eliminate any acute metabolic effects. Strikingly, ß-cells exposed to this high-glucose pulse paradigm exhibited significantly stronger insulin secretion. This metabolic memory was entirely dependent on CaMKII. Metabolic memory was reflected on the protein level by increased expression of proteins involved in glucose sensing and Ca(2+)-dependent vesicle secretion, and by elevated levels of the key ß-cell transcription factor MAFA. In summary, like neurons, human and mouse ß-cells are able to acquire and retrieve information.
Subject: Camkii
Insulin Secretion
Metabolic Memory
Rights: aberto
Identifier DOI: 10.1016/j.molmet.2014.03.011
Address: http://www.ncbi.nlm.nih.gov/pubmed/24944908
Date Issue: 2014
Appears in Collections:Unicamp - Artigos e Outros Documentos

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