Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/201489
Type: Artigo de periódico
Title: A Putative Otu Domain-containing Protein 1 Deubiquitinating Enzyme Is Differentially Expressed In Thyroid Cancer And Identifies Less-aggressive Tumours.
Author: Carneiro, A P
Reis, C F
Morari, E C
Maia, Y C P
Nascimento, R
Bonatto, J M C
de Souza, M A
Goulart, L R
Ward, L S
Abstract: This study aimed to identify novel biomarkers for thyroid carcinoma diagnosis and prognosis. We have constructed a human single-chain variable fragment (scFv) antibody library that was selected against tumour thyroid cells using the BRASIL method (biopanning and rapid analysis of selective interactive ligands) and phage display technology. One highly reactive clone, scFv-C1, with specific binding to papillary thyroid tumour proteins was confirmed by ELISA, which was further tested against a tissue microarray that comprised of 229 thyroid tissues, including: 110 carcinomas (38 papillary thyroid carcinomas (PTCs), 42 follicular carcinomas, 30 follicular variants of PTC), 18 normal thyroid tissues, 49 nodular goitres (NG) and 52 follicular adenomas. The scFv-C1 was able to distinguish carcinomas from benign lesions (P=0.0001) and reacted preferentially against T1 and T2 tumour stages (P=0.0108). We have further identified an OTU domain-containing protein 1, DUBA-7 deubiquitinating enzyme as the scFv-binding antigen using two-dimensional polyacrylamide gel electrophoresis and mass spectrometry. The strategy of screening and identifying a cell-surface-binding antibody against thyroid tissues was highly effective and resulted in a useful biomarker that recognises malignancy among thyroid nodules and may help identify lower-risk cases that can benefit from less-aggressive management.
Subject: Adenocarcinoma, Follicular
Carcinoma, Papillary
Cell Line, Tumor
Diagnosis, Differential
Enzyme-linked Immunosorbent Assay
Humans
Immunoblotting
Peptide Library
Single-chain Antibodies
Thyroid Neoplasms
Tumor Markers, Biological
Ubiquitin-specific Proteases
Rights: fechado
Identifier DOI: 10.1038/bjc.2014.331
Address: http://www.ncbi.nlm.nih.gov/pubmed/24937664
Date Issue: 2014
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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