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Type: Artigo de periódico
Title: Comparison Of The Solution Of Histidine-tryptophan-alfacetoglutarate With Histidine-tryptophan-glutamate As Cardioplegic Agents In Isolated Rat Hearts: An Immunohistochemical Study.
Author: Oliveira, Marcos Aurélio Barboza de
Ferreira, Lívia Carvalho
Zuccari, Débora Aparecida Pires de Campos
Brandi, Antônio Carlos
Santos, Carlos Alberto dos
Botelho, Paulo Henrique Husseni
Petrucci, Orlando
Braile, Domingo Marcolino
Abstract: Cardiac arrest during heart surgery is a common procedure and allows the surgeon to perform surgical procedures in an environment free of blood and movement. Using a model of isolated rat heart, the authors compare a new cardioplegic solution containing histidine-tryptophan-glutamate (group 2) with the histidine-tryptophan-alphacetoglutarate (group 1) routinely used by some cardiac surgeons. To assess caspase, IL-8 and KI-67 in isolated rat hearts using immunohistochemistry. 20 Wistar male rats were anesthetized and heparinized. The chest was opened, cardioctomy was performed and 40 ml/kg of the appropriate cardioplegic solution was infused. The hearts were kept for 2 hours at 4ºC in the same solution, and thereafter, placed in the Langendorff apparatus for 30 minutes with Ringer-Locke solution. Immunohistochemistry analysis of caspase, IL-8, and KI-67 were performed. The concentration of caspase was lower in group 2 and Ki-67 was higher in group 2, both P<0.05. There was no statistical difference between the values of IL-8 between the groups. Histidine-tryptophan-glutamate solution was better than histidine-tryptophan-alphacetoglutarate solution because it reduced caspase (apoptosis), increased KI-67 (cell proliferation), and showed no difference in IL-8 levels compared to group 1. This suggests that the histidine-tryptophan-glutamate solution was more efficient than the histidine-tryptophan-alphacetoglutarate for the preservation of hearts of rat cardiomyocytes.
Subject: Animals
Cardioplegic Solutions
Glutamic Acid
Ki-67 Antigen
Myocytes, Cardiac
Rats, Wistar
Reproducibility Of Results
Time Factors
Citation: Revista Brasileira De Cirurgia Cardiovascular : órgão Oficial Da Sociedade Brasileira De Cirurgia Cardiovascular. v. 29, n. 1, p. 83-8
Rights: aberto
Identifier DOI: 
Date Issue: -1-Uns- -1
Appears in Collections:Unicamp - Artigos e Outros Documentos

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