Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/201449
Full metadata record
DC FieldValueLanguage
dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleDefective Apoptosis In Intestinal And Mesenteric Adipose Tissue Of Crohn's Disease Patients.pt_BR
dc.contributor.authorDias, Cilene Biccapt_BR
dc.contributor.authorMilanski, Marcianept_BR
dc.contributor.authorPortovedo, Marianapt_BR
dc.contributor.authorHorita, Vivianpt_BR
dc.contributor.authorAyrizono, Maria de Lourdes Setsukopt_BR
dc.contributor.authorPlanell, Núriapt_BR
dc.contributor.authorCoy, Cláudio Saddy Rodriguespt_BR
dc.contributor.authorVelloso, Lício Augustopt_BR
dc.contributor.authorMeirelles, Luciana Rodriguespt_BR
dc.contributor.authorLeal, Raquel Francopt_BR
unicamp.authorCilene Bicca Dias, Coloproctology Unit, Surgery Department, University of Campinas (UNICAMP), Medical School, Sao Paulo, Brazilpt_BR
unicamp.authorLaboratory of Cell Signaling, Internal Medicine Department, University of Campinas (UNICAMP), Medical School, Sao Paulo, Brazilpt_BR
unicamp.authorDoctoral CAPES fellowship, Post graduate Program in Surgery Sciences, Faculty of Medical School, University of Campinas, Sao Paulo, Brazil.pt_BR
unicamp.authorMarciane Milanski, Laboratory of Cell Signaling, Internal Medicine Department, University of Campinas (UNICAMP), Medical School, Sao Paulo, Brazil.pt_BR
unicamp.authorMariana Portovedo, Laboratory of Cell Signaling, Internal Medicine Department, University of Campinas (UNICAMP), Medical School, Sao Paulo, Brazil.pt_BR
unicamp.authorVivian Horita, Department of Pathology, University of Campinas (UNICAMP), Medical School, Sao Paulo, Brazil.pt_BR
unicamp.authorMaria de Lourdes Setsuko Ayrizono, Coloproctology Unit, Surgery Department, University of Campinas (UNICAMP), Medical School, Sao Paulo, Brazil.pt_BR
unicamp.authorCláudio Saddy Rodrigues Coy, Coloproctology Unit, Surgery Department, University of Campinas (UNICAMP), Medical School, Sao Paulo, Brazil.pt_BR
unicamp.authorLício Augusto Velloso, Laboratory of Cell Signaling, Internal Medicine Department, University of Campinas (UNICAMP), Medical School, Sao Paulo, Brazil.pt_BR
unicamp.authorLuciana Rodrigues Meirelles, Department of Pathology, University of Campinas (UNICAMP), Medical School, Sao Paulo, Brazil.pt_BR
unicamp.authorRaquel Franco Leal, Coloproctology Unit, Surgery Department, University of Campinas (UNICAMP), Medical School, Sao Paulo, Brazilpt_BR
unicamp.authorLaboratory of Cell Signaling, Internal Medicine Department, University of Campinas (UNICAMP), Medical School, Sao Paulo, Brazil.pt_BR
unicamp.author.externalNúria Planell, Department of Gastroenterology and Bioinformatics Platform, CIBERehd, Barcelona, Spain.pt
dc.subjectAdipose Tissuept_BR
dc.subjectApoptosispt_BR
dc.subjectCase-control Studiespt_BR
dc.subjectCrohn Diseasept_BR
dc.subjectHumanspt_BR
dc.subjectIn Situ Nick-end Labelingpt_BR
dc.subjectIntestinespt_BR
dc.subjectMesenterypt_BR
dc.description.abstractCrohn's disease (CD) is associated with complex pathogenic pathways involving defects in apoptosis mechanisms. Recently, mesenteric adipose tissue (MAT) has been associated with CD ethiopathology, since adipose thickening is detected close to the affected intestinal area. However, the potential role of altered apoptosis in MAT of CD has not been addressed. To evaluate apoptosis in the intestinal mucosa and MAT of patients with CD. Samples of intestinal mucosa and MAT from patients with ileocecal CD and from non-inflammatory bowel diseases patients (controls) were studied. Apoptosis was assessed by TUNEL assay and correlated with the adipocytes histological morphometric analysis. The transcriptional and protein analysis of selected genes and proteins related to apoptosis were determined. TUNEL assay showed fewer apoptotic cells in CD, when compared to the control groups, both in the intestinal mucosa and in MAT. In addition, the number of apoptotic cells (TUNEL) correlated significantly with the area and perimeter of the adipose cells in MAT. Transcriptomic and proteomic analysis reveal a significantly lower transcript and protein levels of Bax in the intestinal mucosa of CD, compared to the controls; low protein levels of Bax were found localized in the lamina propria and not in the epithelium of this tissue. Furthermore, higher level of Bcl-2 and low level of Caspase 3 were seen in the MAT of CD patients. The defective apoptosis in MAT may explain the singular morphological characteristics of this tissue in CD, which may be implicated in the pathophysiology of the disease.en
dc.relation.ispartofPlos Onept_BR
dc.relation.ispartofabbreviationPLoS ONEpt_BR
dc.date.issued2014pt_BR
dc.identifier.citationPlos One. v. 9, n. 6, p. e98547, 2014.pt_BR
dc.language.isoengpt_BR
dc.description.volume9pt_BR
dc.description.firstpagee98547pt_BR
dc.rightsabertopt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn1932-6203pt_BR
dc.identifier.doi10.1371/journal.pone.0098547pt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/24887376pt_BR
dc.date.available2015-11-27T13:42:40Z-
dc.date.accessioned2015-11-27T13:42:40Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T13:42:40Z (GMT). No. of bitstreams: 1 pmed_24887376.pdf: 2457464 bytes, checksum: 06bccfc9f71debcd1e3e941e0e2a52c7 (MD5) Previous issue date: 2014en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/201449-
dc.identifier.idPubmed24887376pt_BR
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File SizeFormat 
pmed_24887376.pdf2.4 MBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.