Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/201442
Type: Artigo de periódico
Title: Antioxidant And Antiproliferative Activities Of Methanolic Extract From A Neglected Agricultural Product: Corn Cobs.
Author: Melo-Silveira, Raniere Fagundes
Fidelis, Gabriel Pereira
Viana, Rony Lucas Silva
Soeiro, Vinícius Campelo
Silva, Rodrigo Augusto da
Machado, Daisy
Costa, Leandro Silva
Ferreira, Carmen Veríssima
Oliveira Rocha, Hugo Alexandre
Abstract: Neglected agricultural products (NAPs) are defined as discarded material in agricultural production. Corn cobs are a major waste of agriculture maize. Here, a methanolic extract from corn cobs (MEC) was obtained. MEC contains phenolic compounds, protein, carbohydrates (1.4:0.001:0.001). We evaluated the in vitro and in vivo antioxidant potential of MEC. Furthermore, its antiproliferative property against tumor cells was assessed through MTT assays and proteins related to apoptosis in tumor cells were examined by western blot. MEC showed no hydroxyl radical scavenger capacity, but it showed antioxidant activity in Total Antioxidant Capacity and DPPH scavenger ability assays. MEC showed higher Reducing Power than ascorbic acid and exhibited high Superoxide Scavenging activity. In tumor cell culture, MEC increased catalase, metallothionein and superoxide dismutase expression in accordance with the antioxidant tests. In vivo antioxidant test, MEC restored SOD and CAT, decreased malondialdehyde activities and showed high Trolox Equivalent Antioxidant Capacity in animals treated with CCl4. Furthermore, MEC decreased HeLa cells viability by apoptosis due an increase of Bax/Bcl-2 ratio, caspase 3 active. Protein kinase C expression increased was also detected in treated tumor cells. Thus, our findings pointed out the biotechnological potential of corn cobs as a source of molecules with pharmacological activity.
Subject: Animals
Antineoplastic Agents, Phytogenic
Apoptosis
Catalase
Cell Proliferation
Cell Survival
Drug Screening Assays, Antitumor
Free Radical Scavengers
Hela Cells
Humans
Inhibitory Concentration 50
Metallothionein
Methanol
Oxidation-reduction
Plant Components, Aerial
Plant Extracts
Rats, Wistar
Solid Phase Extraction
Solvents
Superoxide Dismutase
Superoxides
Zea Mays
Rights: aberto
Identifier DOI: 10.3390/molecules19045360
Address: http://www.ncbi.nlm.nih.gov/pubmed/24879583
Date Issue: 2014
Appears in Collections:Unicamp - Artigos e Outros Documentos

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