Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/201439
Type: Artigo de periódico
Title: Neutralisation Of The Pharmacological Activities Of Bothrops Alternatus Venom By Anti-pla2 Iggs.
Author: Garcia Denegri, María E
Maruñak, Silvana
Todaro, Juan S
Ponce-Soto, Luis A
Acosta, Ofelia
Leiva, Laura
Abstract: Basic phospholipases A2 (PLA2) are toxic and induce a wide spectrum of pharmacological effects, although the acidic enzyme types are not lethal or cause low lethality. Therefore, it is challenging to elucidate the mechanism of action of acidic phospholipases. This study used the acidic non-toxic Ba SpII RP4 PLA2 from Bothrops alternatus as an antigen to develop anti-PLA2 IgG antibodies in rabbits and used in vivo assays to examine the changes in crude venom when pre-incubated with these antibodies. Using Ouchterlony and western blot analyses on B. alternatus venom, we examined the specificity and sensitivity of phospholipase A2 recognition by the specific antibodies (anti-PLA2 IgG). Neutralisation assays using a non-toxic PLA2 antigen revealed unexpected results. The (indirect) haemolytic activity of whole venom was completely inhibited, and all catalytically active phospholipases A2 were blocked. Myotoxicity and lethality were reduced when the crude venom was pre-incubated with anti-PLA2 immunoglobulins. CK levels in the skeletal muscle were significantly reduced at 6 h, and the muscular damage was more significant at this time-point compared to 3 and 12 h. When four times the LD50 was used (224 μg), half the animals treated with the venom-anti PLA2 IgG mixture survived after 48 h. All assays performed with the specific antibodies revealed that Ba SpII RP4 PLA2 had a synergistic effect on whole-venom toxicity. IgG antibodies against the venom of the Argentinean species B. alternatus represent a valuable tool for elucidation of the roles of acidic PLA2 that appear to have purely digestive roles and for further studies on immunotherapy and snake envenoming in affected areas in Argentina and Brazil.
Subject: Animals
Antibodies, Anti-idiotypic
Antibodies, Neutralizing
Antibody Specificity
Antivenins
Bothrops
Crotalid Venoms
Immunoglobulin G
Male
Mice
Neutralization Tests
Phospholipase A2 Inhibitors
Phospholipases A2
Rabbits
Anti-pla(2) Immunoglobulins
Bothrops Alternatus
Envenomation
Non-myotoxic Pla(2)
Rights: fechado
Identifier DOI: 10.1016/j.toxicon.2014.05.016
Address: http://www.ncbi.nlm.nih.gov/pubmed/24878372
Date Issue: 2014
Appears in Collections:Unicamp - Artigos e Outros Documentos

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