Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/201431
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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampVettorazzi, Jean Franciescopt_BR
dc.contributor.authorunicampSantos-Silva, Junia Carolina Rebelopt_BR
dc.contributor.authorunicampBorck, Patricia Cristinept_BR
dc.contributor.authorunicampBatista, Thiago Martinspt_BR
dc.contributor.authorunicampNardelli, Tarlliza Romannapt_BR
dc.contributor.authorunicampBoschiero, Antonio Carlospt_BR
dc.contributor.authorunicampCarneiro, Everardo Magalhãespt_BR
dc.typeArtigopt_BR
dc.titleTaurine supplementation increases K(atp) channel protein content, improving Ca2+ handling and insulin secretion in islets from malnourished mice fed on a high-fat dietpt_BR
dc.contributor.authorVettorazzi, Jean F.pt_BR
dc.contributor.authorRibeiro, Rosane A.pt_BR
dc.contributor.authorSantos-Silva, Junia C.pt_BR
dc.contributor.authorBorck, Patricia C.pt_BR
dc.contributor.authorBatista, Thiago M.pt_BR
dc.contributor.authorNardelli, Tarlliza R.pt_BR
dc.contributor.authorBoschero, Antonio C.pt_BR
dc.contributor.authorCarneiro, Everardo M.pt_BR
dc.contributor.authorpt_BR
unicamp.authorJean F Vettorazzi, Laboratório de Pâncreas Endócrino e Metabolismo, Departamento de Biologia Estrutural e Funcional, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil.pt_BR
unicamp.author.externalRosane A Ribeiro,pt
unicamp.author.externalJunia C Santos-Silva,pt
unicamp.author.externalPatricia C Borck,pt
unicamp.author.externalThiago M Batista,pt
unicamp.author.externalTarlliza R Nardelli,pt
unicamp.author.externalAntonio C Boschero,pt
unicamp.author.externalEverardo M Carneiro,pt
dc.subjectDieta hiperlipídicapt_BR
dc.subjectInsulina - Secreçãopt_BR
dc.subjectCanais KATPpt_BR
dc.subjectDesnutrição proteicapt_BR
dc.subjectTaurinapt_BR
dc.subjectCanais de Cálciopt_BR
dc.subject.otherlanguageDiet, high-fatpt_BR
dc.subject.otherlanguageInsulin - Secretionpt_BR
dc.subject.otherlanguageKATP channelspt_BR
dc.subject.otherlanguageProtein malnutritionpt_BR
dc.subject.otherlanguageTaurinept_BR
dc.subject.otherlanguageCalcium channelspt_BR
dc.description.abstractPancreatic β-cells are highly sensitive to suboptimal or excess nutrients, as occurs in protein-malnutrition and obesity. Taurine (Tau) improves insulin secretion in response to nutrients and depolarizing agents. Here, we assessed the expression and function of Cav and KATP channels in islets from malnourished mice fed on a high-fat diet (HFD) and supplemented with Tau. Weaned mice received a normal (C) or a low-protein diet (R) for 6 weeks. Half of each group were fed a HFD for 8 weeks without (CH, RH) or with 5% Tau since weaning (CHT, RHT). Isolated islets from R mice showed lower insulin release with glucose and depolarizing stimuli. In CH islets, insulin secretion was increased and this was associated with enhanced KATP inhibition and Cav activity. RH islets secreted less insulin at high K(+) concentration and showed enhanced KATP activity. Tau supplementation normalized K(+)-induced secretion and enhanced glucose-induced Ca(2+) influx in RHT islets. R islets presented lower Ca(2+) influx in response to tolbutamide, and higher protein content and activity of the Kir6.2 subunit of the KATP. Tau increased the protein content of the α1.2 subunit of the Cav channels and the SNARE proteins SNAP-25 and Synt-1 in CHT islets, whereas in RHT, Kir6.2 and Synt-1 proteins were increased. In conclusion, impaired islet function in R islets is related to higher content and activity of the KATP channels. Tau treatment enhanced RHT islet secretory capacity by improving the protein expression and inhibition of the KATP channels and enhancing Synt-1 islet content.en
dc.description.abstractPancreatic β-cells are highly sensitive to suboptimal or excess nutrients, as occurs in protein-malnutrition and obesity. Taurine (Tau) improves insulin secretion in response to nutrients and depolarizing agents. Here, we assessed the expression and functpt_BR
dc.relation.ispartofAmino acids: the forum for amino acid, peptide and protein researchpt_BR
dc.publisher.cityWienpt_BR
dc.publisher.countryAustriapt_BR
dc.publisherSpringerpt_BR
dc.date.issued2014pt_BR
dc.date.monthofcirculationSept.pt_BR
dc.identifier.citationAmino Acids. v. 46, n. 9, p. 2123-36, 2014-Sep.pt_BR
dc.language.isoengpt_BR
dc.description.volume46pt_BR
dc.description.issuenumber9pt_BR
dc.description.firstpage2123pt_BR
dc.description.lastpage2136pt_BR
dc.rightsfechadopt_BR
dc.rightsfechadopt_br
dc.sourcePUBMEDpt_BR
dc.identifier.issn0939-4451pt_BR
dc.identifier.eissn1438-2199pt_BR
dc.identifier.doi10.1007/s00726-014-1763-6pt_BR
dc.identifier.urlhttps://link.springer.com/article/10.1007%2Fs00726-014-1763-6pt_BR
dc.description.sponsorshipFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOpt_BR
dc.description.sponsorshipCNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOpt_BR
dc.description.sponsorshipCAPES - COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIORpt_BR
dc.description.sponsorship1FAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOpt_BR
dc.description.sponsorship1CNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOpt_BR
dc.description.sponsorship1CAPES - COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIORpt_BR
dc.description.sponsordocumentnumbersem informaçãopt_BR
dc.description.sponsordocumentnumbersem informaçãopt_BR
dc.description.sponsordocumentnumbersem informaçãopt_BR
dc.date.available2015-11-27T13:42:37Z-
dc.date.accessioned2015-11-27T13:42:37Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T13:42:37Z (GMT). No. of bitstreams: 1 pmed_24866813.pdf: 1069407 bytes, checksum: 9b5f3b9c30e82bd66a02125437946945 (MD5) Previous issue date: 2014 Bitstreams deleted on 2020-10-14T14:31:56Z: pmed_24866813.pdf,. Added 1 bitstream(s) on 2020-10-14T14:35:52Z : No. of bitstreams: 1 24866813.pdf: 1144469 bytes, checksum: 4c444d0f91d4dda870e95e4bf3666243 (MD5)en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/201431-
dc.identifier.idPubmed24866813pt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentDepartamento de Biologia Estrutural e Funcionalpt_BR
dc.contributor.departmentDepartamento de Biologia Estrutural e Funcionalpt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.subject.keywordHigh-fat dietpt_BR
dc.subject.keywordInsulin secretionpt_BR
dc.subject.keywordTaurine supplementationpt_BR
dc.subject.keywordVoltage-sensitive Ca2+ channelspt_BR
dc.identifier.source24866813pt_BR
dc.creator.orcidsem informaçãopt_BR
dc.creator.orcid0000-0002-8667-8140pt_BR
dc.creator.orcidsem informaçãopt_BR
dc.creator.orcid0000-0001-6971-1191pt_BR
dc.creator.orcid0000-0002-4643-1098pt_BR
dc.creator.orcid0000-0003-3829-8570pt_BR
dc.creator.orcid0000-0003-3212-369Xpt_BR
dc.type.formArtigo originalpt_BR
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