Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/201430
Type: Artigo de periódico
Title: Desquamation Is A Novel Phenomenon For Collective Prostate Epithelial Cell Deletion After Castration.
Author: Rosa-Ribeiro, Rafaela
Barbosa, Guilherme Oliveira
Kühne, Fabiana
Carvalho, Hernandes F
Abstract: The mechanism underlying castration-induced prostate regression, which is a classical physiological concept translated into the therapeutic treatment of advanced prostate cancer, involves epithelial cell apoptosis. In searching for events and mechanisms contributing to prostate regression in response to androgen modulation, we have frequently observed the collective deletion of epithelial cells. This work was undertaken to characterize this phenomenon hereafter named desquamation and to verify its presence after 17β-estradiol (E2) administration. Electron microscopy revealed that the desquamating cells had preserved cell-cell junctions and collapsed nuclear contents. The TUNEL reaction was negative for these cells, which were also negative for cleaved caspases-8, -9, -3 and nuclear apoptosis-inducing factor. Detailed analyses revealed that the condensed chromatin was first affected detaching from the nuclear lamina, which was observable after lamin A immunohistochemistry, suggesting the lack of lamin A degradation. A search in animals treated with supraphysiological E2 employed as an alternative anti-androgen treatment revealed no desquamation. The combined treatment (Cas + E2 group) caused changes particular to each treatment, including desquamation. In conclusion, desquamation appeared as a novel phenomenon contributing to collective prostate epithelial cell deletion, distinct from the classical castration-induced apoptosis and particular to the androgen deprivation resulting from surgical castration, and should be considered as part of the mechanisms promoting organ regression.
Subject: Animals
Castration
Cell Differentiation
Eosine Yellowish-(ys)
Epithelial Cells
Hematoxylin
Immunohistochemistry
Male
Prostate
Rats
Rats, Wistar
Rights: fechado
Identifier DOI: 10.1007/s00418-013-1152-3
Address: http://www.ncbi.nlm.nih.gov/pubmed/24105629
Date Issue: 2014
Appears in Collections:Unicamp - Artigos e Outros Documentos

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