Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/201428
Type: Artigo de periódico
Title: Carbon Nanoparticles For Gene Transfection In Eukaryotic Cell Lines.
Author: Zanin, H
Hollanda, L M
Ceragioli, H J
Ferreira, M S
Machado, D
Lancellotti, M
Catharino, R R
Baranauskas, V
Lobo, A O
Abstract: For the first time, oxygen terminated cellulose carbon nanoparticles (CCN) was synthesised and applied in gene transfection of pIRES plasmid. The CCN was prepared from catalytic of polyaniline by chemical vapour deposition techniques. This plasmid contains one gene that encodes the green fluorescent protein (GFP) in eukaryotic cells, making them fluorescent. This new nanomaterial and pIRES plasmid formed π-stacking when dispersed in water by magnetic stirring. The frequencies shift in zeta potential confirmed the plasmid strongly connects to the nanomaterial. In vitro tests found that this conjugation was phagocytised by NG97, NIH-3T3 and A549 cell lines making them fluorescent, which was visualised by fluorescent microscopy. Before the transfection test, we studied CCN in cell viability. Both MTT and Neutral Red uptake tests were carried out using NG97, NIH-3T3 and A549 cell lines. Further, we use metabolomics to verify if small amounts of nanomaterial would be enough to cause some cellular damage in NG97 cells. We showed two mechanisms of action by CCN-DNA complex, producing an exogenous protein by the transfected cell and metabolomic changes that contributed by better understanding of glioblastoma, being the major finding of this work. Our results suggested that this nanomaterial has great potential as a gene carrier agent in non-viral based therapy, with low cytotoxicity, good transfection efficiency, and low cell damage in small amounts of nanomaterials in metabolomic tests.
Subject: 3t3 Cells
Animals
Carbon
Cell Line, Tumor
Cell Survival
Cellulose
Dna
Green Fluorescent Proteins
Humans
Metabolomics
Mice
Microscopy, Electron, Scanning
Nanoparticles
Particle Size
Plasmids
Spectroscopy, Fourier Transform Infrared
Spectrum Analysis, Raman
Transfection
Carbon Nanomaterial
Cell Viability
Gene Transfection
Metabolomic Test
Rights: fechado
Identifier DOI: 10.1016/j.msec.2014.03.016
Address: http://www.ncbi.nlm.nih.gov/pubmed/24863237
Date Issue: 2014
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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