Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/201427
Type: Artigo de periódico
Title: Graphene And Carbon Nanotube Nanocomposite For Gene Transfection.
Author: Hollanda, L M
Lobo, A O
Lancellotti, M
Berni, E
Corat, E J
Zanin, H
Abstract: Graphene and carbon nanotube nanocomposite (GCN) was synthesised and applied in gene transfection of pIRES plasmid conjugated with green fluorescent protein (GFP) in NIH-3T3 and NG97 cell lines. The tips of the multi-walled carbon nanotubes (MWCNTs) were exfoliated by oxygen plasma etching, which is also known to attach oxygen content groups on the MWCNT surfaces, changing their hydrophobicity. The nanocomposite was characterised by high resolution scanning electron microscopy; energy-dispersive X-ray, Fourier transform infrared and Raman spectroscopies, as well as zeta potential and particle size analyses using dynamic light scattering. BET adsorption isotherms showed the GCN to have an effective surface area of 38.5m(2)/g. The GCN and pIRES plasmid conjugated with the GFP gene, forming π-stacking when dispersed in water by magnetic stirring, resulting in a helical wrap. The measured zeta potential confirmed that the plasmid was connected to the nanocomposite. The NIH-3T3 and NG97 cell lines could phagocytize this wrap. The gene transfection was characterised by fluorescent protein produced in the cells and pictured by fluorescent microscopy. Before application, we studied GCN cell viability in NIH-3T3 and NG97 line cells using both MTT and Neutral Red uptake assays. Our results suggest that GCN has moderate stability behaviour as colloid solution and has great potential as a gene carrier agent in non-viral based therapy, with low cytotoxicity and good transfection efficiency.
Subject: 3t3 Cells
Adsorption
Animals
Cell Line, Tumor
Cell Survival
Graphite
Green Fluorescent Proteins
Humans
Magnetics
Mice
Microscopy, Electron, Scanning
Nanocomposites
Nanotubes, Carbon
Transfection
Water
Carbon Nanotubes
Cell Viability
Composite
Gene Transfer
Graphene Oxide
Plasma
Rights: fechado
Identifier DOI: 10.1016/j.msec.2014.03.002
Address: http://www.ncbi.nlm.nih.gov/pubmed/24863227
Date Issue: 2014
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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