Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/201371
Type: Artigo de periódico
Title: Dna Extraction From Filter-paper Spots Of Vaginal Samples Collected After Sexual Violence.
Author: Campos, Elisabete A
Pitta, Denise R
Costa, Fernanda A
Campos, Vanessa M
Yela, Daniela
Fernandes, Arlete
Abstract: To detect the presence of male DNA in vaginal samples collected from survivors of sexual violence and stored on filter paper. A pilot study was conducted to evaluate 10 vaginal samples spotted on sterile filter paper: 6 collected at random in April 2009 and 4 in October 2010. Time between sexual assault and sample collection was 4-48hours. After drying at room temperature, the samples were placed in a sterile envelope and stored for 2-3years until processing. DNA extraction was confirmed by polymerase chain reaction for human β-globin, and the presence of prostate-specific antigen (PSA) was quantified. The presence of the Y chromosome was detected using primers for sequences in the TSPY (Y7/Y8 and DYS14) and SRY genes. β-Globin was detected in all 10 samples, while 2 samples were positive for PSA. Half of the samples amplified the Y7/Y8 and DYS14 sequences of the TSPY gene and 30% amplified the SRY gene sequence of the Y chromosome. Four male samples and 1 female sample served as controls. Filter-paper spots stored for periods of up to 3years proved adequate for preserving genetic material from vaginal samples collected following sexual violence.
Subject: Adolescent
Adult
Chromosomes, Human, Y
Dna
Female
Humans
Kallikreins
Male
Pilot Projects
Prostate-specific Antigen
Sex Offenses
Specimen Handling
Time Factors
Vagina
Young Adult
Dna
Filter Paper
Sexual Violence
Rights: fechado
Identifier DOI: 10.1016/j.ijgo.2014.02.010
Address: http://www.ncbi.nlm.nih.gov/pubmed/24792405
Date Issue: 2014
Appears in Collections:Unicamp - Artigos e Outros Documentos

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