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Type: Artigo de periódico
Title: Pipkiiα Is Widely Expressed In Hematopoietic-derived Cells And May Play A Role In The Expression Of Alpha- And Gamma-globins In K562 Cells.
Author: Peretti de Albuquerque Wobeto, Vânia
Machado-Neto, João Agostinho
Zaccariotto, Tânia Regina
Ribeiro, Daniela Maria
da Silva Santos Duarte, Adriana
Saad, Sara Teresinha Olalla
Costa, Fernando Ferreira
de Fatima Sonati, Maria
Abstract: Characterized for the first time in erythrocytes, phosphatidylinositol phosphate kinases (PIP kinases) belong to a family of enzymes that generate various lipid messengers and participate in several cellular processes, including gene expression regulation. Recently, the PIPKIIα gene was found to be differentially expressed in reticulocytes from two siblings with hemoglobin H disease, suggesting a possible relationship between PIPKIIα and the production of globins. Here, we investigated PIPKIIα gene and protein expression and protein localization in hematopoietic-derived cells during their differentiation, and the effects of PIPKIIα silencing on K562 cells. PIPKIIα silencing resulted in an increase in α and γ globins and a decrease in the proliferation of K562 cells without affecting cell cycle progression and apoptosis. In conclusion, using a cell line model, we showed that PIPKIIα is widely expressed in hematopoietic-derived cells, is localized in their cytoplasm and nucleus, and is upregulated during erythroid differentiation. We also showed that PIPKIIα silencing can induce α and γ globin expression and decrease cell proliferation in K562 cells.
Subject: Apoptosis
Cell Proliferation
Gene Expression Regulation
Gene Silencing
Hematopoietic Stem Cells
K562 Cells
Phosphotransferases (alcohol Group Acceptor)
Citation: Molecular And Cellular Biochemistry. v. 393, n. 1-2, p. 145-53, 2014-Aug.
Rights: fechado
Identifier DOI: 10.1007/s11010-014-2054-y
Date Issue: 2014
Appears in Collections:Unicamp - Artigos e Outros Documentos

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