Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/201325
Type: Artigo de periódico
Title: Haemophilus Influenzae Porine Ompp2 Gene Transfer Mediated By Graphene Oxide Nanoparticles With Effects On Transformation Process And Virulence Bacterial Capacity.
Author: Varela, Julia Nogueira
Amstalden, Maria Cecília Krähenbühl
Pereira, Rafaella Fabiana Carneiro
de Hollanda, Luciana Maria
Ceragioli, Helder José
Baranauskas, Vitor
Lancellotti, Marcelo
Abstract: H. influenzae is a natural competent bacterium that can uptake DNA from the environment and recombine into bacterial genome. The outbreaks of Brazilian purpuric fever, heavily polluted areas of a different H. influenzae biogroup - aegyptius - as well as gene transference between Neisseria meningitis make the transformation process an important evolutionary factor. This work studied the horizontal transference of the ompP2 gene from a multiresistant strain of H. influenzae 07 (NTHi), under the influence of graphene oxide nanoparticles in order to mimic an atmosphere rich in suspended particles and this way verify if the CFU transformants number was increased. In this article the gene ompP2 was transformed into different strains of H. influenzae mediated or not by graphene oxide nanoparticles in suspension, followed by the adhesion tests in Hec-1B (human endometrium adenocarcinoma) and A549 (pulmonary epithelial carcinoma) cells lines. The transformation frequency and the adhesion capacity were determined in all the mutants to which the ompP2 gene was transferred and compared to their wild type strains. The nanoparticles increased the transformation ratio of one particular strain isolated from a pneumonia case. The adhesion patterns to A549 and Hec1b cell lines of these mutated bacteria has their capacity increased when compared to the wild type. Graphene oxide nanoparticles aid the transformation process, helping to increase the number of CFUs, and the mutants generated with the ompP2 gene from a H. influenzae resistant strain not only present a chloramphenicol resistance but also have an increased adherence patterns in A549 and Hec1B cell lines.
Subject: Bacterial Adhesion
Bacterial Proteins
Cell Line, Tumor
Graphite
Haemophilus Infections
Haemophilus Influenzae
Host-pathogen Interactions
Humans
Mutation
Nanoparticles
Oxides
Porins
Transformation, Bacterial
Rights: aberto
Identifier DOI: 10.1186/1477-3155-12-14
Address: http://www.ncbi.nlm.nih.gov/pubmed/24739467
Date Issue: 2014
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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