Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/201282
Type: Artigo de periódico
Title: Differential Srna Regulation In Leaves And Roots Of Sugarcane Under Water Depletion.
Author: Thiebaut, Flávia
Grativol, Clícia
Tanurdzic, Milos
Carnavale-Bottino, Mariana
Vieira, Tauan
Motta, Mariana Romeiro
Rojas, Cristian
Vincentini, Renato
Chabregas, Sabrina Moutinho
Hemerly, Adriana Silva
Martienssen, Robert A
Ferreira, Paulo Cavalcanti Gomes
Abstract: Plants have developed multiple regulatory mechanisms to respond and adapt to stress. Drought stress is one of the major constraints to agricultural productivity worldwide and recent reports have highlighted the importance of plant sRNA in the response and adaptation to water availability. In order to increase our understanding of the roles of sRNA in response to water depletion, cultivars of sugarcane were submitted to treatment of ceasing drip irrigation for 24 hours. Deep sequencing analysis was carried out to identify the sRNA regulated in leaves and roots of sugarcane cultivars with different drought sensitivities. The pool of sRNA selected allowed the analysis of different sRNA classes (miRNA and siRNA). Twenty-eight and 36 families of conserved miRNA were identified in leaf and root libraries, respectively. Dynamic regulation of miRNA was observed and the expression profiles of eight miRNA were verified in leaf samples from three biological replicates by stem-loop qRT-PCR assay using the cultivars: SP90-1638--sensitive cultivar--and SP83-2847 and SP83-5073--tolerant cultivars. Altered miRNA regulation was correlated with changes in mRNA levels of specific targets. Two leaf libraries from individual sugarcane cultivars with contrasting drought-tolerance properties were also analyzed. An enrichment of 22-nt sRNA species was observed in leaf libraries. 22-nt miRNA triggered siRNA production by cleavage of their targets in response to water depletion. A number of genes of the sRNA biogenesis pathway were down-regulated in tolerant genotypes and up-regulated in sensitive in response to water depletion treatment. Our analysis contributes to increase the knowledge on the roles of sRNA in sugarcane submitted to water depletion.
Subject: Droughts
Gene Expression Regulation, Plant
Plant Leaves
Plant Roots
Rna, Plant
Saccharum
Stress, Physiological
Rights: aberto
Identifier DOI: 10.1371/journal.pone.0093822
Address: http://www.ncbi.nlm.nih.gov/pubmed/24695493
Date Issue: 2014
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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