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Type: Artigo de periódico
Title: Avian Extraintestinal Escherichia Coli Exhibits Enterotoxigenic-like Activity In The In Vivo Rabbit Ligated Ileal Loop Assay.
Author: Maluta, Renato Pariz
Gatti, Maria Silvia Viccari
Joazeiro, Paulo Pinto
de Paiva, Jacqueline Boldrin
Rojas, Thaís Cabrera Galvão
Silveira, Flávio
Houle, Sébastien
Kobayashi, Renata Katsuko Takayama
Dozois, Charles M
Dias da Silveira, Wanderley
Abstract: Avian pathogenic Escherichia coli (APEC) strains harbor a number of virulence genes and cause extraintestinal diseases, such as septicemia, swollen-head syndrome, salpingitis, and omphalitis in poultry. APEC strains are not known to cause intestinal diseases. Herein, for the first time, it is reported that APEC strains were able to induce an enterotoxigenic-like effect in rabbit ligated ileal loops. Strain SEPT362 caused cell detachment of the intestinal villi, which also showed a flattened and wilted appearance, but the integrity of the tight junctions was maintained. Additionally, this strain did not adhere to enterocytes in vivo, although adhesin encoding genes ( fimH, csgA, lpfA2-3, and ECP) were present while other lpfA types, sfa, afa, papC, and ral genes were not. This enterotoxigenic-like activity was conserved after thermal treatment of the supernatant at 65°C but not at 100°C. Moreover, experiments based on filtering with different molecular weight cut-off (MWCO) pore sizes demonstrated that the component associated with the observed biological effect has a molecular weight >100 kDa. Blast search and polymerase chain reaction assays for known E. coli virulence factors showed that strain SEPT362 harbors the gene encoding for the toxin EAST-1 and the serine protease autotransporter (SPATE) Tsh, but is negative for genes encoding for the toxins LT-I, STh, STp, Stx1, Stx2, CNF-1, CNF-2, CDT and the SPATEs Sat, Pic, Vat, SigA, SepA, EatA, EspP, or EspC. A cloned copy of the tsh gene in E. coli K-12 was also tested and was shown to have an enterotoxic effect. These results suggest that APEC might induce fluid accumulation in the rabbit gut. The Tsh autotransporter seems to be one of the factors associated with this phenotype.
Subject: Adhesins, Escherichia Coli
Bacterial Adhesion
Enterotoxigenic Escherichia Coli
Escherichia Coli Infections
Hot Temperature
In Vitro Techniques
Intestinal Mucosa
Poultry Diseases
Recombinant Proteins
Virulence Factors
Water-electrolyte Imbalance
Rights: fechado
Identifier DOI: 10.1089/fpd.2013.1719
Date Issue: 2014
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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