Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/201249
Type: Artigo de periódico
Title: Maternal High-fat Diet Consumption Modulates Hepatic Lipid Metabolism And Microrna-122 (mir-122) And Microrna-370 (mir-370) Expression In Offspring.
Author: Benatti, R O
Melo, A M
Borges, F O
Ignacio-Souza, L M
Simino, L A P
Milanski, M
Velloso, L A
Torsoni, M A
Torsoni, A S
Abstract: Maternal consumption of a high-fat diet (HFD) during pregnancy and lactation is closely related to hepatic lipid accumulation, insulin resistance and increased serum cytokine levels in offspring and into their adulthood. MicroRNA (miRNA) have been implicated in cholesterol biosynthesis and fatty acid metabolism. We evaluated the modulation of hepatic fatty acid synthesis (de novo), β-oxidation pathways, and miRNA-122 (miR-122) and miRNA-370 (miR-370) expression in recently weaned offspring (day 28) of mouse dams fed a HFD (HFD-O) or a standard chow (SC-O) during pregnancy and lactation. Compared with SC-O mice, HFD-O mice weighed more, had a larger adipose tissue mass and were more intolerant to glucose and insulin (P< 0·05). HFD-O mice also presented more levels of serum cholesterol, TAG, NEFA and hepatic IκB kinase and c-Jun N-terminal kinase phosphorylation compared with SC-O mice (P< 0·05). Protein levels of fatty acid synthase, acetyl-CoA carboxylase and 3-hydroxy-3-methylglutaryl-CoA reductase were similar in HFD-O and SC-O mice, whereas expression levels of SCD1 mRNA and protein were more abundant in HFD-O mice than in SC-O mice (P< 0·05). Interestingly, mRNA expression levels of the β-oxidation-related genes ACADVL and CPT1 were decreased in HFD-O mice (P< 0·05). Furthermore, the expression of miR-122 was reduced but that of miR-370 was increased in HFD-O mice compared with that in SC-O mice (P< 0·05). Changes in hepatic lipid metabolism were accompanied by increased mRNA content of AGPAT1 and TAG deposition in HFD-O mice (P< 0·05). Taken together, the present results strongly suggest that maternal consumption of a HFD affects the early lipid metabolism of offspring by modulating the expression of hepatic β-oxidation-related genes and miRNA that can contribute to metabolic disturbances in adult life.
Subject: Adiposity
Animals
Diet, High-fat
Female
Fetal Development
Gene Expression Regulation, Developmental
Glucose Intolerance
Lactation
Lipid Metabolism
Liver
Male
Maternal Nutritional Physiological Phenomena
Mice
Micrornas
Obesity
Pregnancy
Pregnancy Complications
Random Allocation
Specific Pathogen-free Organisms
Weaning
Weight Gain
Rights: fechado
Identifier DOI: 10.1017/S0007114514000579
Address: http://www.ncbi.nlm.nih.gov/pubmed/24666709
Date Issue: 2014
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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