Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/201228
Type: Artigo de periódico
Title: Expanding Resolution Of Metalloprotein Separations From Soybean Seeds Using 2d-hplc-icp-ms And Sds-page As A Third Dimension.
Author: Mataveli, Lidiane Raquel Verola
Arruda, Marco Aurélio Zezzi
Abstract: This work reports on the use of a three dimensional separation system to enhance metalloprotein information when considering soybean seeds. Separations using size exclusion chromatography (SEC) allowed identification of three metal fractions. Following an anion exchange (AEX) chromatographic separation in the second dimension, the resultant sub-fractions were lyophilized and subjected to a third dimension of separation using a polyacrylamide gel electrophoresis (SDS-PAGE). After the separation, the bands were digested, and, in addition to others, the following proteins, previously associated with metals, were identified: 3-lipoxygenase A chain (soybean) complex with 13(S)-hydroperoxy-9(Z),11(E)-octadecadienoic acid, beta-amylase [Glycine max], seed lipoxygenase-1, lipoxygenase [G. max], seed lipoxygenase-2 (Pisum sativum) and beta-conglycinin. Techniques presenting high resolution are desired due to their capability in resolving great amount of signals (responses) generated from hundreds of proteins generally found in different samples. To the best of our knowledge, this is the first time that bidimensional chromatographic system which allied to another separation dimension is applied for improving protein identification, so that higher number and different proteins were found when comparing 2D dimension with 3D dimension. In fact, this strategy is welcoming in proteomics studies, in order to improve the comprehension of those systems that present large number of proteins. This article is part of a Special Issue entitled: Environmental and structural proteomics.
Subject: Chromatography, High Pressure Liquid
Electrophoresis, Polyacrylamide Gel
Metalloproteins
Proteome
Reproducibility Of Results
Seeds
Sensitivity And Specificity
Soybeans
Spectrometry, Mass, Electrospray Ionization
Analytical Proteomics
Mass Spectrometry
Metalloproteins
Separations
Soybean
Rights: fechado
Identifier DOI: 10.1016/j.jprot.2014.02.031
Address: http://www.ncbi.nlm.nih.gov/pubmed/24631827
Date Issue: 2014
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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