Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/201180
Type: Artigo de periódico
Title: New Hydrocarbon Degradation Pathways In The Microbial Metagenome From Brazilian Petroleum Reservoirs.
Author: Sierra-García, Isabel Natalia
Correa Alvarez, Javier
de Vasconcellos, Suzan Pantaroto
Pereira de Souza, Anete
dos Santos Neto, Eugenio Vaz
de Oliveira, Valéria Maia
Abstract: Current knowledge of the microbial diversity and metabolic pathways involved in hydrocarbon degradation in petroleum reservoirs is still limited, mostly due to the difficulty in recovering the complex community from such an extreme environment. Metagenomics is a valuable tool to investigate the genetic and functional diversity of previously uncultured microorganisms in natural environments. Using a function-driven metagenomic approach, we investigated the metabolic abilities of microbial communities in oil reservoirs. Here, we describe novel functional metabolic pathways involved in the biodegradation of aromatic compounds in a metagenomic library obtained from an oil reservoir. Although many of the deduced proteins shared homology with known enzymes of different well-described aerobic and anaerobic catabolic pathways, the metagenomic fragments did not contain the complete clusters known to be involved in hydrocarbon degradation. Instead, the metagenomic fragments comprised genes belonging to different pathways, showing novel gene arrangements. These results reinforce the potential of the metagenomic approach for the identification and elucidation of new genes and pathways in poorly studied environments and contribute to a broader perspective on the hydrocarbon degradation processes in petroleum reservoirs.
Subject: Aerobiosis
Bacteria
Brazil
Hydrocarbons
Metagenome
Petroleum
Phylogeny
Synteny
Rights: aberto
Identifier DOI: 10.1371/journal.pone.0090087
Address: http://www.ncbi.nlm.nih.gov/pubmed/24587220
Date Issue: 2014
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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