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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleEvaluation Of Fructooligosaccharides Separation Using A Fixed-bed Column Packed With Activated Charcoal.pt_BR
dc.contributor.authorKuhn, Raquel Cristinept_BR
dc.contributor.authorMazutti, Marcio Apt_BR
dc.contributor.authorAlbertini, Lilian Buoropt_BR
dc.contributor.authorFilho, Francisco Maugeript_BR
unicamp.authorLilian Buoro Albertini, Bioprocess Engineering Laboratory, Food Engineering Department, University of Campinas - UNICAMP, Campinas, SP, CEP 13083-862, Brazil.pt_BR
unicamp.authorFrancisco Maugeri Filho, Bioprocess Engineering Laboratory, Food Engineering Department, University of Campinas - UNICAMP, Campinas, SP, CEP 13083-862, Brazil.pt_BR
unicamp.author.externalRaquel Cristine Kuhn, Department of Chemical Engineering, Federal University of Santa Maria, Av. Roraima, 1000, Santa Maria, RS, CEP 97105-900, Brazil. Electronic address: raquelckuhn@yahoo.com.br.pt
unicamp.author.externalMarcio A Mazutti, Department of Chemical Engineering, Federal University of Santa Maria, Av. Roraima, 1000, Santa Maria, RS, CEP 97105-900, Brazil.pt
dc.subjectCharcoalpt_BR
dc.subjectChromatography, High Pressure Liquidpt_BR
dc.subjectGlucosept_BR
dc.subjectOligosaccharidespt_BR
dc.subjectTime Factorspt_BR
dc.description.abstractRecent studies have shown that the chromatographic separation of mixtures of saccharides may be improved by making use of activated charcoal, a promising low cost material for the separation of sugars, including fructooligosaccharides. In this work, the development of a methodology to separate fructooligosaccharides from glucose, fructose and sucrose, using a fixed bed column packed with activated charcoal is proposed. The influence of temperature, eluant concentration and step gradients were evaluated to increase the separation efficiency and fructooligosaccharide purity. The final degree of fructooligosaccharide purification and separation efficiency were about 94% and 3.03 respectively, using ethanol gradient concentration ranging from 3.5% to 15% (v/v) at 40°C. The fixed bed column packed with the activated charcoal was shown to be a promising alternative for sugar separation, mainly those rich in fructooligosaccharides, leading to solutions of acceptable degrees of purification.en
dc.relation.ispartofNew Biotechnologypt_BR
dc.relation.ispartofabbreviationN Biotechnolpt_BR
dc.date.issued2014-Maypt_BR
dc.identifier.citationNew Biotechnology. v. 31, n. 3, p. 237-41, 2014-May.pt_BR
dc.language.isoengpt_BR
dc.description.volume31pt_BR
dc.description.firstpage237-41pt_BR
dc.rightsfechadopt_BR
dc.rights.holderCopyright © 2014 Elsevier B.V. All rights reserved.pt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn1876-4347pt_BR
dc.identifier.doi10.1016/j.nbt.2014.02.005pt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/24583465pt_BR
dc.date.available2015-11-27T13:42:01Z-
dc.date.accessioned2015-11-27T13:42:01Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T13:42:01Z (GMT). No. of bitstreams: 1 pmed_24583465.pdf: 229715 bytes, checksum: a893bfb50c3c27bb6096ff1e489cb875 (MD5) Previous issue date: 2014en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/201179-
dc.identifier.idPubmed24583465pt_BR
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