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Type: Artigo de periódico
Title: Transcriptome Profile Of Trichoderma Harzianum Ioc-3844 Induced By Sugarcane Bagasse.
Author: Horta, Maria Augusta Crivelente
Vicentini, Renato
Delabona, Priscila da Silva
Laborda, Prianda
Crucello, Aline
Freitas, Sindélia
Kuroshu, Reginaldo Massanobu
Polikarpov, Igor
Pradella, José Geraldo da Cruz
Souza, Anete Pereira
Abstract: Profiling the transcriptome that underlies biomass degradation by the fungus Trichoderma harzianum allows the identification of gene sequences with potential application in enzymatic hydrolysis processing. In the present study, the transcriptome of T. harzianum IOC-3844 was analyzed using RNA-seq technology. The sequencing generated 14.7 Gbp for downstream analyses. De novo assembly resulted in 32,396 contigs, which were submitted for identification and classified according to their identities. This analysis allowed us to define a principal set of T. harzianum genes that are involved in the degradation of cellulose and hemicellulose and the accessory genes that are involved in the depolymerization of biomass. An additional analysis of expression levels identified a set of carbohydrate-active enzymes that are upregulated under different conditions. The present study provides valuable information for future studies on biomass degradation and contributes to a better understanding of the role of the genes that are involved in this process.
Subject: Cellulase
Databases, Genetic
Gene Expression Profiling
Genes, Fungal
Molecular Sequence Annotation
Sequence Analysis, Rna
Citation: Plos One. v. 9, n. 2, p. e88689, 2014.
Rights: aberto
Identifier DOI: 10.1371/journal.pone.0088689
Date Issue: 2014
Appears in Collections:Unicamp - Artigos e Outros Documentos

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