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Type: Artigo de periódico
Title: Endothelial Activation By Platelets From Sickle Cell Anemia Patients.
Author: Proença-Ferreira, Renata
Brugnerotto, Ana Flávia
Garrido, Vanessa Tonin
Dominical, Venina Marcela
Vital, Daiana Morelli
Ribeiro, Marilene de Fátima Reis
dos Santos, Melissa Ercolin
Traina, Fabíola
Olalla-Saad, Sara T
Costa, Fernando Ferreira
Conran, Nicola
Abstract: Sickle cell anemia (SCA) is associated with a hypercoagulable state. Increased platelet activation is reported in SCA and SCA platelets may present augmented adhesion to the vascular endothelium, potentially contributing to the vaso-occlusive process. We sought to observe the effects of platelets (PLTs) from healthy control (CON) individuals and SCA individuals on endothelial activation, in vitro. Human umbilical vein endothelial cells (HUVEC) were cultured, in the presence, or not, of washed PLTs from CON or steady-state SCA individuals. Supernatants were reserved for cytokine quantification, and endothelial adhesion molecules (EAM) were analyzed by flow cytometry; gene expressions of ICAM1 and genes of the NF-κB pathway were analyzed by qPCR. SCA PLTs were found to be more inflammatory, displaying increased adhesive properties, an increased production of IL-1β and a tendency towards elevated expressions of P-selectin and activated αIIbβ3. Following culture in the presence of SCA PLTs, HUVEC presented significant augmentations in the expressions of the EAM, ICAM-1 and E-selectin, as well as increased IL-8 production and increased ICAM1 and NFKB1 (encodes p50 subunit of NF-κB) gene expressions. Interestingly, transwell inserts abolished the effects of SCA PLTs on EAM expression. Furthermore, an inhibitor of the NF-κB pathway, BAY 11-7082, also prevented the induction of EAM expression on the HUVEC surface by SCA PLTs. In conclusion, we find further evidence to indicate that platelets circulate in an activated state in sickle cell disease and are capable of stimulating endothelial cell activation. This effect appears to be mediated by direct contact, or even adhesion, between the platelets and endothelial cells and via NFκB-dependent signaling. As such, activated platelets in SCD may contribute to endothelial activation and, therefore, to the vaso-occlusive process. Results provide further evidence to support the use of anti-platelet approaches in association with other therapies for SCD.
Subject: Adolescent
Anemia, Sickle Cell
Blood Platelets
Cell Adhesion
Coculture Techniques
Gene Expression Regulation
Human Umbilical Vein Endothelial Cells
Intercellular Adhesion Molecule-1
Middle Aged
Nf-kappa B
Platelet Glycoprotein Gpiib-iiia Complex
Primary Cell Culture
Signal Transduction
Rights: aberto
Identifier DOI: 10.1371/journal.pone.0089012
Date Issue: 2014
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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