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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleVegf Receptor Expression Decreases During Lung Development In Congenital Diaphragmatic Hernia Induced By Nitrofen.pt_BR
dc.contributor.authorSbragia, Lpt_BR
dc.contributor.authorNassr, A C Cpt_BR
dc.contributor.authorGonçalves, F L Lpt_BR
dc.contributor.authorSchmidt, A Fpt_BR
dc.contributor.authorZuliani, C Cpt_BR
dc.contributor.authorGarcia, P Vpt_BR
dc.contributor.authorGallindo, R Mpt_BR
dc.contributor.authorPereira, L A Vpt_BR
unicamp.authorC C Zuliani, Departamento de Clínica Médica, Faculdade de Ciências Médicas, Universidade Estadual de Campinas, CampinasSP, Brasil, Departamento de Clínica Médica, Faculdade de Ciências Médicas, Universidade Estadual de Campinas, Campinas, SP, Brasil.pt_BR
unicamp.authorP V Garcia, Departamento de Histologia e Embriologia, Instituto de Biologia, Universidade Estadual de Campinas, UNICAMP, CampinasSP, Brasil, Departamento de Histologia e Embriologia, Instituto de Biologia, Universidade Estadual de Campinas, UNICAMP, Campinas, SP, Brasil.pt_BR
unicamp.authorL A V Pereira, Departamento de Histologia e Embriologia, Instituto de Biologia, Universidade Estadual de Campinas, UNICAMP, CampinasSP, Brasil, Departamento de Histologia e Embriologia, Instituto de Biologia, Universidade Estadual de Campinas, UNICAMP, Campinas, SP, Brasil.pt_BR
unicamp.author.externalL Sbragia, Divisão de Cirurgia Pediátrica, Departamento de Cirurgia e Anatomia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão PretoSP, Brasil, Divisão de Cirurgia Pediátrica, Departamento de Cirurgia e Anatomia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil.pt
unicamp.author.externalA C C Nassr, Departamento de Hidrobiologia do Centro de Ciências Biológicas e da Saúde, Universidade Federal de São Carlos, São CarlosSP, Brasil, Departamento de Hidrobiologia do Centro de Ciências Biológicas e da Saúde, Universidade Federal de São Carlos, São Carlos, SP, Brasil.pt
unicamp.author.externalF L L Gonçalves, Divisão de Cirurgia Pediátrica, Departamento de Cirurgia e Anatomia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão PretoSP, Brasil, Divisão de Cirurgia Pediátrica, Departamento de Cirurgia e Anatomia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil.pt
unicamp.author.externalA F Schmidt, Pediatrics House Office, Cincinnati Children's Hospital Medical Center, CincinnatiOH, USA, Pediatrics House Office, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA.pt
unicamp.author.externalR M Gallindo, Divisão de Cirurgia Pediátrica, Departamento de Cirurgia e Anatomia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão PretoSP, Brasil, Divisão de Cirurgia Pediátrica, Departamento de Cirurgia e Anatomia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil.pt
dc.subjectAnimalspt_BR
dc.subjectDisease Models, Animalpt_BR
dc.subjectFemalept_BR
dc.subjectHernias, Diaphragmatic, Congenitalpt_BR
dc.subjectLungpt_BR
dc.subjectPhenyl Etherspt_BR
dc.subjectPregnancypt_BR
dc.subjectRats, Sprague-dawleypt_BR
dc.subjectReceptors, Vascular Endothelial Growth Factorpt_BR
dc.description.abstractChanges in vascular endothelial growth factor (VEGF) in pulmonary vessels have been described in congenital diaphragmatic hernia (CDH) and may contribute to the development of pulmonary hypoplasia and hypertension; however, how the expression of VEGF receptors changes during fetal lung development in CDH is not understood. The aim of this study was to compare morphological evolution with expression of VEGF receptors, VEGFR1 (Flt-1) and VEGFR2 (Flk-1), in pseudoglandular, canalicular, and saccular stages of lung development in normal rat fetuses and in fetuses with CDH. Pregnant rats were divided into four groups (n=20 fetuses each) of four different gestational days (GD) 18.5, 19.5, 20.5, 21.5: external control (EC), exposed to olive oil (OO), exposed to 100 mg nitrofen, by gavage, without CDH (N-), and exposed to nitrofen with CDH (CDH) on GD 9.5 (term=22 days). The morphological variables studied were: body weight (BW), total lung weight (TLW), left lung weight, TLW/BW ratio, total lung volume, and left lung volume. The histometric variables studied were: left lung parenchymal area density and left lung parenchymal volume. VEGFR1 and VEGFR2 expression were determined by Western blotting. The data were analyzed using analysis of variance with the Tukey-Kramer post hoc test. CDH frequency was 37% (80/216). All the morphological and histometric variables were reduced in the N- and CDH groups compared with the controls, and reductions were more pronounced in the CDH group (P<0.05) and more evident on GD 20.5 and GD 21.5. Similar results were observed for VEGFR1 and VEGFR2 expression. We conclude that N- and CDH fetuses showed primary pulmonary hypoplasia, with a decrease in VEGFR1 and VEGFR2 expression.en
dc.relation.ispartofBrazilian Journal Of Medical And Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas / Sociedade Brasileira De Biofísica ... [et Al.]pt_BR
dc.relation.ispartofabbreviationBraz. J. Med. Biol. Res.pt_BR
dc.date.issued2014-Febpt_BR
dc.identifier.citationBrazilian Journal Of Medical And Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas / Sociedade Brasileira De Biofísica ... [et Al.]. v. 47, n. 2, p. 171-8, 2014-Feb.pt_BR
dc.language.isoengpt_BR
dc.description.volume47pt_BR
dc.description.firstpage171-8pt_BR
dc.rightsabertopt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn1414-431Xpt_BR
dc.identifier.doi10.1590/1414-431X20133221pt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/24519134pt_BR
dc.date.available2015-11-27T13:41:54Z-
dc.date.accessioned2015-11-27T13:41:54Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T13:41:54Z (GMT). No. of bitstreams: 1 pmed_24519134.pdf: 1972789 bytes, checksum: 757b8965ed6581b6ba29111c10868deb (MD5) Previous issue date: 2014en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/201136-
dc.identifier.idPubmed24519134pt_BR
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